草莓Trihelix转录因子家族的鉴定及表达分析OA北大核心CSTPCD

Trihelix are transcription factors that can specifically bind to the light-responsive element GT element,and are widely involved in plant growth and development and response to adversity stress.In this study,the Trihelix transcription factor family members were identified in the strawberry(Fragaria vesca)genome,and further conducted bioinformatics and expression analysis under stress.The results showed that the strawberry Trihelix transcription factor family consisted of 26 members,distributed on chr01～chr07,among them,the largest number of members were distributed on chr06,with 7 Trihelix transcription factor family members.The results of the subcellular localization prediction showed that the FvTrihelix transcription factor family members were mainly localized in the nucleus and cytoplasm.Phylogenetic analysis showed that FvTrihelix proteins were divided into 5 subfamilies,of which Group2,Group3,Group4 and Group5,contained 1,3,11 and 11 FvTrihelix members,respectively.Cis-acting element analysis showed that the members of this family were mainly related to exogenous hormones,drought and low temperature response elements.qRT-PCR results showed that the relative expression of FvTrihelix14 were significantly higher than the control under 4℃condition(P＜0.05),being 8 times that of the control(CK).The expression level of FvTrihelix15 under 10%PEG and 4℃treatments showed significant differences compared to the CK(P＜0.05),being 12 and 14 times that of the control,respectively.In summary,the members of the strawberry Trihelix transcription factor family are able to respond to various stress conditions.This study provides a theoretical basis for the screening and application of stress-resistant genes in strawberries.