农业生物技术学报2024，Vol.32Issue(9)：2049-2059,11.DOI:10.3969/j.issn.1674-7968.2024.09.008
金鱼草GAE基因家族鉴定及核盘菌抗性基因挖掘
Identification of GAE Gene Family in Antirrhinum majus and Mining of Resistance Genes to Sclerotinia sclerotiorum
摘要
Abstract
Pectin,a crucial constituent of plant cell walls,assumes a significant role in both plant development and cell wall fortification.The enzymatic catalyst responsible for pectin polymerization is glucuronate isomerase,also known as UDP-D-glucuronate-4-epimerase(GAE).This enzymatic process involves the utilization of UDP-α-D-galacturonic acid(UDP-GalA)as the monosaccharide donor to form the pectin skeleton.To investigate the cell wall resistance mediated by GAE of Antirrhinum majus,8 AmGAEs genes were identified at the whole genome level.Bioinformatics analysis of their physical and chemical properties,subcellular localization,phylogeny,conserved motifs,gene structure,chromosome localization and cis-acting elements showed that all the members of the AmGAE family were alkaline hydrophilic proteins located in Golgi apparatus.The genes had resistance-related response elements such as defense and stress,methyljasmonate(MeJA),salicylic acid(SA)and abscisic acid(ABA)in the promoter region.Furthermore,RNA-seq and qRT-PCR were used to analyse and reverify the resistant and susceptible materials to S.sclerotiorum in snapdragon.The results showed that the expression of AmGAE1a,AmGAE3,AmGAE5 and AmGAE2 genes were significantly induced in the resistant material Am6(R),while the expression of AmGAE7 and AmGAE4 genes was significantly induced in the susceptible material Am1(S).Among them,the expression patterns of AmGAE1a,AmGAE3,AmGAE7 and AmGAE4 genes in qRT-PCR were consistent with differentially expressed genes(DEGs)by RNA-seq,and thus finally identified as candidate genes.The results provide a theoretical basis and new genetic resources of AmGAE family genes involved in the resistance to S.sclerotiorum for further study.关键词
金鱼草/GAE基因家族/核盘菌抗性/转录组分析/候选基因挖掘Key words
Antirrhinum majus/GAE gene family/Resistance to Sclerotinia sclerotiorum/Transcriptome analysis/Candidate gene mining分类
农业科技引用本文复制引用
夏文念,杨冬梅,宋佳怡,杨洁,汪仲毅,蔺海娣,胡慧贞..金鱼草GAE基因家族鉴定及核盘菌抗性基因挖掘[J].农业生物技术学报,2024,32(9):2049-2059,11.基金项目
国家自然科学基金(31901571) （31901571）
西南林业大学科研启动项目(01102-112109) （01102-112109）
云南省省级大学生创新创业训练计划(202110677078) （202110677078）
