农业生物技术学报2024，Vol.32Issue(9)：2124-2136,13.DOI:10.3969/j.issn.1674-7968.2024.09.015
辣椒根际促生菌的筛选及促生效应分析
Screening of Rhizosphere Growth-promoting Bacteria of Pepper(Capsicum annuum)and Analysis of Their Beneficial Effects
摘要
Abstract
Excessive application of chemical fertilizers and pesticides and soil continuous cropping have seriously restricted the production of pepper(Capsicum annuum),so it is very important to improve the soil environment so as to increase the yield of pepper.In order to screen rhizosphere growth-promoting bacteria of pepper and study their promoting effect,the rhizosphere soil from pepper in Qiqihar city was used as experimental materials.The growth-promoting bacteria in the rhizosphere of pepper was screened out using functional media,and identified by 16S rDNA sequence.The seed germination and pot experiments were conducted to verify the growth-promoting effect of the strains with strong growth-promoting ability.A total of 51 strains of pepper rhizosphere growth-promoting bacteria were isolated,which belonged to 12 genera.The results showed that among the 51 growth-promoting bacteria,8 strains had the ability of phosphorus resolving,35 strains could produce gibberellin,33 strains could secrete auxin(IAA),and all 51 strains had the ability of phosphorus-solubilizing,potassium releasing and nitrogen fixation.Bacillus tequilensis K10 could significantly promote pepper seed germination and increased the number of root hairs of pepper young roots.B.siamensis G19-1 and Acinetobacter johnsonii N9-2 significantly increased above ground fresh weight,root fresh weight,aboveground dry weight,root dry weight and plant height(P＜0.05),and the ability of bacteria to solubilize phosphorus,release potassium,fix nitrogen,produce gibberellins and IAA was significantly positively correlated with aboveground fresh weight,aboveground dry weight,and root dry weight of pepper plants(P＜0.05).In conclusion,this experiment screened 3 growth-promoting bacteria K10,G19-1 and N9-2 as candidate strains for pepper microbial fertilizer,and will provide strain resources and technical support for the development of pepper microbial fertilizer.关键词
辣椒/分离/促生效应/根际促生菌Key words
Capsicum annuum/Isolation/Growth promoting effect/Plant growth promoting rhizobacteria分类
农业科技引用本文复制引用
张琳,余红凤,毕钰,王志刚,徐伟慧,刘桂琳..辣椒根际促生菌的筛选及促生效应分析[J].农业生物技术学报,2024,32(9):2124-2136,13.基金项目
齐齐哈尔市食品产业研究院项目(SXSP-2021004) （SXSP-2021004）
黑龙江省属本科高校基本科研业务费成果转化后补助项目(145209808) （145209808）
