农业生物技术学报2024，Vol.32Issue(9)：2137-2149,13.DOI:10.3969/j.issn.1674-7968.2024.09.016
自噬相关基因PlATG12对荔枝霜疫霉生长发育和致病性的影响
Effect of Autophagy-related Gene PlATG12 on the Growth,Development and Pathogenicity of Peronophythora litchii
摘要
Abstract
Autophagy plays an important role in plant resistance to pathogen infection.The autophagy-related protein 12(ATG12),is mainly involved in the formation and extension of the double-layer membrane of autophagy.However,its role in plant pathogens,especially Oomycetes,has been rarely reported.In order to clarify the role of Peronophythora litchii PlATG12(GeneBank No.OR909654)in the growth and development,and pathogenesis,the PlATG12 knockout mutants were obtained by CRISPR/Cas9 gene editing and PEG mediated protoplast transformation technology,and the in situ complementation was performed.Phenotypic assay results showed that compared with the wild-type strain SHS3(WT),the average mycelial growth rate of the ΔPlatg12-77 and ΔPlatg12-315 mutants was decreased by 6.50%and 7.49%,respectively,and the average sporangium number was reduced by 33.86%and 34.02%,respectively.Meanwhile,the zoospore release rate of the mutants was significantly lower than those of WT after incubating at 12℃for 0.5 and 2 h.In addition,the oospore production was only 20.25%and 22.00%of the WT,respectively,and the oospore of typeⅢand type Ⅳ increased significantly.The pathogenicity of PlATG12 knockout mutants on litchi(Litchi chinensis)leaves was lower than that of WT.However,the phenotypic defects of PlATG12 knockout mutants were rescued after in situ complementation of PlATG12.These results indicated that autophagy-related gene PlATG12 plays a key role in the growth and development,and pathogenesis of P.litchii.This study provides theoretical basis for the functions of autophagy pathway in the pathogenic process of oomycetes.关键词
荔枝霜疫霉/细胞自噬/荔枝霜疫霉自噬相关蛋白12(PlATG12)/生长发育/致病性Key words
Peronophythora litchii/Autophagy/P.litchii autophagy-related protein 12(PlATG12)/Growth and development/Pathogenicity分类
农业科技引用本文复制引用
陈泰旭,杨成东,于戈,罗曼菲,张雪,吕林,陈庆河..自噬相关基因PlATG12对荔枝霜疫霉生长发育和致病性的影响[J].农业生物技术学报,2024,32(9):2137-2149,13.基金项目
海南省自然科学基金创新研究团队项目(321CXTD437) （321CXTD437）
国家自然科学基金(32160614) （32160614）
海南大学科研启动基金[KYQD(ZR)-20080] （ZR）
