农业生物技术学报2024，Vol.32Issue(9)：2173-2180,8.DOI:10.3969/j.issn.1674-7968.2024.09.019
山羊睾丸类器官的培养与鉴定
Culture and Characterization of Testicular Organoid in Goats(Capra hircus)
摘要
Abstract
Organoid is a tissue substitute that is structurally and functionally similar to the origin organ.At present,many organoid culture systems have been established,however testicular organoid culture is still in the exploratory stage.In this study,testicular tissues of Saanen dairy goats(Capra hircus)were taken,and testicular cells were isolated and cultured by mixed enzyme digestion.Testicular cells were cultured in three dimensions using a mixed medium containing low melting point agarose.The formed organoids were evaluated using light microscope and immunofluorescence detection techniques,and the culture effects of different sources of testicular cells,different numbers of testicular cells and different concentrations of low melting point agarose on testicular organoids were compared.Finally,the model function of the testicular organoid was verified by lipopolysaccharide(LPS)infection.The result showed that cells ranging from 2×105 to 3×105,in 1%low-melting-point agarose were more favorable for the formation of goat testicular organs in goats.The organoids formed possess seminiferous tubule-like structures,with spermatogonial stem cells and Sertoli cells evenly distributed within.Testicular organoid infected with LPS were smaller than normal organoids,exhibited cellular debris,and were accompanied by a significant increase in inflammatory factors,indicating that these organoids could respond to external stimuli and simulate the immune response of organs to a certain extent,making them a suitable model for the in vitro study of testicular diseases.This study not only promotes the development of testicular organoid culture technology,but also provides an important scientific basis and experimental platform for a variety of fields,including reproductive biology,disease modeling,drug screening,regenerative medicine,and personalized medicine.关键词
睾丸类器官/低熔点琼脂糖胶/山羊Key words
Testicular organoid/Low melting point agarose gels/Goat分类
农业科技引用本文复制引用
徐文静,吴文萍,杨玉梅,李娜,华进联..山羊睾丸类器官的培养与鉴定[J].农业生物技术学报,2024,32(9):2173-2180,8.基金项目
国家自然科学基金(32372970 （）
32072806) （）
国家重点研发计划(2023YFF100904) （2023YFF100904）
陕西省农业核心技术攻关(2024NYGG005-6 （）
20230978) （）
内蒙古自治区揭榜挂帅项目(2022JBGUS0025) （2022JBGUS0025）
