农业生物技术学报2024，Vol.32Issue(9)：2181-2189,9.DOI:10.3969/j.issn.1674-7968.2024.09.020
变形假单胞菌免疫刺激复合物对大黄鱼口服免疫效果的评价
Evaluation of the Oral Immune Effects of Pseudomonas plecoglossicida Immune Stimulating Complexes on Large Yellow Croaker(Larimichthys crocea)
摘要
Abstract
Pseudomonas plecoglossicida is the main pathogen which causes the visceral white spot disease in large yellow croaker(Larimichthys crocea),leading to serious economic loss.Previous studies have shown that immune stimulating complexes(ISCOMs)have good oral immune effects.To evaluate the oral immune effects of P.plecoglossicida immune stimulating complexes(Pp-ISCOMs)on L.crocea,Pp-ISCOMs vaccine was prepared,which presented a cage-like nanoparticle structure.At 21 d post oral vaccination,the expression of immune-related genes in the head-kidney of immunized L.crocea were detected,and the immune serum titer was measured using agglutination tests.The immune protective effects of Pp-ISCOMs were then determined by challenge assay with P.plecoglossicida.The results showed that the expression of immune or anti-inflammatory factors such as myeloid differentiation factor 88(MyD88)(P＜0.05),interleukin 2(IL-2)(P＜0.001)in the head kidney of Pp-ISCOMs-immunized L.crocea were significantly higher than those of the inactived-P.plecoglossicida-immunized group(Pp)or the blank control group(CK);and the agglutination titer of large yellow croaker serum(21 d post immunization)was 22.After a P.plecoglossicida attack with a dose of 5×102 CFU/g by intraperitoneal injection,the relative percent survival(RPS)of L.crocea vaccinated by Pp-ISCOMs and Pp were estimated to be 73.3%and 40%respectively.The results indicate that the Pp-ISCOMs could improve the resistance of L.crocea to P.plecoglossicida,and suggested a promising prospect for application.This study provides technical support for the prevention and control by immune to visceral white spot disease in L.crocea.关键词
变形假单胞菌/免疫刺激复合物(ISCOMs)/口服/大黄鱼/免疫效果Key words
Pseudomonas plecoglossicida/Immune stimulating complexes(ISCOMs)/Oral/Larimichthys crocea/Immune effect分类
农业科技引用本文复制引用
许斌福,陈秀霞,池洪树,李素一,郑在予,徐梦婷,江秋欢,郑炜强,林能锋..变形假单胞菌免疫刺激复合物对大黄鱼口服免疫效果的评价[J].农业生物技术学报,2024,32(9):2181-2189,9.基金项目
国家重点研发计划(2022YFD2401002) （2022YFD2401002）
福建省重大专项专题(2021NZ033025) （2021NZ033025）
福建省公益专项专题(2022R1027005 （）
2023R1025006) （）
宁德市揭榜挂帅项目(ND2023J005) （ND2023J005）
