中国海洋大学学报（自然科学版）2024，Vol.54Issue(9)：70-77,8.DOI:10.16441/j.cnki.hdxb.20220329
两种青岛沿岸常见江蓠科物种室内培养过程中附生菌群的组成及其变化分析
Analysis of the Composition and Changes of Epiphytic Flora in the Indoor Culture of Two Common Species of Gracilariaceae at Qingdao Coast
摘要
Abstract
The growth status of macroalgae collected in the wild is often not as good as that in the wild after a period of indoor cultivation.To answer the question whether the composition and number of the epiphytic flora would change during this progress,two closed related species in Gracilariaceae,Gracilaria vermiculophylla and Gracilariopsis lemaneiformis were collected from Qingdao coast.These two species shared similar morphology and distributed nearly.The bacteria compositions and changes were analyzed and compared for these two macroalgae species during the 90 days indoor cultiva-tion.Firstly,the study evaluated the suitability of 16S rDNA specific primers(799F-1 392R)for analy-zing the epiphytic flora of Gracilaria vvermiculophylla and Gracilariopsis lemaneiformis.The primer pair 799F-1 392R was found to be effective in avoiding interference from the chloroplasts of these algae,enabling a smooth analysis of the flora structure.Next,16S rDNA sequencing was used to analyze the community diversity,community structure,and changes in the epiphytic flora of Gracilaria vermiculo-phylla and Gracilariopsis lemaneiformis.The results revealed differences between the epiphytic flora of the algae in their natural habitat and those cultured indoors.The richness of the epiphytic flora was lower in the indoor culture of Gracilariaceae compared to the wild environment.Additionally,there were differences in the composition of epiphytic flora between Gracilaria vermiculophylla and Gracilariopsis lemaneiformis,with various types of epiphytic flora showing different preferences for these two species of Gracilariaceae.The study suggested that the enormous differences between the indoor and natural en-vironments might be the main reason for the changes of epiphytic flora.Furthermore,the variation in chemical composition between the two Gracilariaceae species also influenced the bacteria attached to their surfaces.Overall,this study provides theoretical support for enhancing our understanding of the relation-ship between algae and bacteria,as well as optimizing the indoor culture of algae.关键词
真江蓠/龙须菜/附生菌/16SrDNA测序/菌群变化Key words
Gracilaria vermiculophylla/Gracilariopsis lemaneiformis/epiphytic bacteria/16S rDNA sequencing/flora change分类
农业科技引用本文复制引用
齐聪,薄志文,梁恩源,杜启睿,徐涤..两种青岛沿岸常见江蓠科物种室内培养过程中附生菌群的组成及其变化分析[J].中国海洋大学学报（自然科学版）,2024,54(9):70-77,8.基金项目
宁波市科技创新2025重大专项(2019B10009) （2019B10009）
现代农业产业技术体系专项资金项目(CARS-50)资助 Supported by the Key Program of Science and Technology Innovation 2025 in Ningbo(2019B10009) （CARS-50）
the Funding program in China Agriculture Research System(CARS-50) （CARS-50）
