中国海洋大学学报（自然科学版）2024，Vol.54Issue(9)：88-95,8.DOI:10.16441/j.cnki.hdxb.20220497
基于转录组的假微型海链藻硫代谢相关途径分析
Transcriptome-Based Analysis of Sulfur Metabolism-Related Pathways in Thalassiosira pseudonana
摘要
Abstract
In order to provide an in-depth analysis of the regulating strategies for cellular processes of key life metabolic activities in the offshore widespread algal bloom species,when they are facing exten-sive salt variations,the transcriptomic sequencing for Thalassiosira pseudonana under different salini-ties and different cultivation time was executed in this study,and expression of sulfur metabolism relat-ed genes was analyzed.A total of 13 differentially expressed genes that related to sulfur metabolic path-ways were identified with different salinity.The number of differentially expressed functional genes gradually increased in the reducing-salinity experimental group compared to the control group over cul-turing time.For example,during sulfur assimilation,the gene expression of ATP-sulfurylase was sig-nificantly lower on the second day of incubation compared to the first day,probably due to reducing de-mand for sulfate assimilation in the cells,which would further limit sulfite formation.Besides,the gene expression related to cysteine synthesis(e.g.serine acetyltransferase)significantly decreased in low salt stress.These results showed that the synthesis of sulfur-containing amino acids(such as cysteine bio-synthesis)in T.pseudonana was reduced,and thus influencing the metabolic activity of microalgae.This study demonstrates that salinity fluctuation in the coastal estuarine areas will potentially affect the sulfur metabolism of T.pseudonana,which is helpful to understand the molecular mechanism of off-shore widespread algal bloom species in response to salinity stress.关键词
假微型海链藻/硫代谢/转录组/盐度/差异性表达基因Key words
Thalassiosira pseudonana/sulfur metabolism/transcriptome/salinity/differentially expressed gene分类
生物科学引用本文复制引用
李佳岷,孙文杰,王华龙,米铁柱,甄毓..基于转录组的假微型海链藻硫代谢相关途径分析[J].中国海洋大学学报（自然科学版）,2024,54(9):88-95,8.基金项目
青岛海洋科学与技术试点国家实验室科技创新项目(LSKJ202203700) （LSKJ202203700）
国家自然科学基金项目(41976133,42206147)资助 Supported by the Science and Technology Innovation Project of the Pilot National Laboratory for Marine Science and Technology(Qingdao)(LSKJ202203700) （41976133,42206147）
the National Natural Science Foundation of China(41976133,42206147) （41976133,42206147）
