强激光与粒子束2024，Vol.36Issue(9)：17-24,8.DOI:10.11884/HPLPB202436.240128
基于相位恢复的透明样本3维重建系统
Three-dimensional reconstruction system for transparent samples based on phase retrieval
摘要
Abstract
In response to the difficulties posed by traditional microscopy imaging techniques in capturing the structure and thickness of colorless transparent samples,we have designed a miniature three-dimensional reconstruction system for such samples.This innovative system,breaking away from traditional optical structures,performs phase retrieval on transparent samples to achieve three-dimensional reconstruction.It requires only light carrying sample information,which is then bifurcated by a spectroscope and captured by a stereo camera.Constructed using 3D printing technology,the compact system measures just 110 mm×110 mm×60 mm,offering a cost-effective solution that is also compatible with traditional microscopy imaging equipment.It incorporates autofocus and field of view correction algorithms,which,by collecting one over-focused and one under-focused image,solve the transport intensity equation to enable phase retrieval and hence the three-dimensional reconstruction of transparent samples.Test results have shown that the system can achieve an imaging resolution of 2.46 μm under a 10×objective lens,and the phase recovery accuracy can also meet the basic requirements.Furthermore,the successful three-dimensional reconstruction of blood cells and scratches on microscope slides validates the system's feasibility and practicality.关键词
显微成像/3维重建/3D打印/光强传输方程/相位恢复Key words
microscopy imaging/three-dimensional reconstruction/3D printing/transport intensity equation/phase retrieval分类
数理科学引用本文复制引用
曹皓月,赵晨,刘静,彭贺,周扬,杨树蔚,马悦,卢奕冰..基于相位恢复的透明样本3维重建系统[J].强激光与粒子束,2024,36(9):17-24,8.基金项目
陕西省教育厅2023年度一般专项科学研究计划项目(23JK0586) （23JK0586）
陕西省自然科学基础研究计划项目(2018JQ2075) （2018JQ2075）
