基于相位恢复的透明样本3维重建系统OA北大核心CSTPCD

In response to the difficulties posed by traditional microscopy imaging techniques in capturing the structure and thickness of colorless transparent samples,we have designed a miniature three-dimensional reconstruction system for such samples.This innovative system,breaking away from traditional optical structures,performs phase retrieval on transparent samples to achieve three-dimensional reconstruction.It requires only light carrying sample information,which is then bifurcated by a spectroscope and captured by a stereo camera.Constructed using 3D printing technology,the compact system measures just 110 mm×110 mm×60 mm,offering a cost-effective solution that is also compatible with traditional microscopy imaging equipment.It incorporates autofocus and field of view correction algorithms,which,by collecting one over-focused and one under-focused image,solve the transport intensity equation to enable phase retrieval and hence the three-dimensional reconstruction of transparent samples.Test results have shown that the system can achieve an imaging resolution of 2.46 μm under a 10×objective lens,and the phase recovery accuracy can also meet the basic requirements.Furthermore,the successful three-dimensional reconstruction of blood cells and scratches on microscope slides validates the system's feasibility and practicality.