山东科学2024，Vol.37Issue(4)：17-25,9.DOI:10.3976/j.issn.1002-4026.20230165
黄连琥珀清心丸质量标准研究
A study on the quality standards of Huanglian Hupo Qingxin pills
摘要
Abstract
To establish a method for the qualitative and quantitative assay of Huanglian Hupo Qingxin pills and provide abasis for the subsequent establishment of quality standards,the six ingredients in the formula(Aquilariae Lignum Resinatum,Fructus Choerospondiatis,Terminalia Chebula,CoptidisRhizoma,Flos Gossampini,and Semen Myristicae)were identified through microscopical observation.Thin-layer chromatography was used to identify Terminalia Chebula.Berberine hydrochloride concentration was assessed using high-performance liquid chromatography.Microscopical and thin-layer identifications of Huanglian Hupo Qingxin pills were conducted,which revealed a berberine hydrochloride concentration of 0.018 0～0.902 0 mg/mL,exhibiting a good linear relationship.The assay method was found to bescientific,sensitive,and reproducible,with relative standard deviations(RSDs)for precision,stability,and reproducibility tests all below 3.0%.The average recovery rate from spike-and-recovery testing was 101.29%,with an RSD of 2.30%.The established quality standardis simple and reproducible and can be used for studying the quality standards of Huanglian Hupo Qingxin pills.关键词
黄连琥珀清心丸/质量标准/显微鉴别/薄层色谱/高效液相色谱法Key words
Huanglian Hupo Qingxin pills/quality standards/microscopical identification/thin-layer chromatography/high-performance liquid chromatography分类
中医学引用本文复制引用
张潇丹,赵渤年,贺晓东,高燕,杨龙飞,张冠群,于子翔,柳明君,王雅菲,更藏加..黄连琥珀清心丸质量标准研究[J].山东科学,2024,37(4):17-25,9.基金项目
山东省重点研发计划项目(2021CXGC010511) （2021CXGC010511）
