山东医药2024，Vol.64Issue(21)：35-38,4.DOI:10.3969/j.issn.1002-266X.2024.21.008
姜黄素对人视网膜色素上皮细胞中基质金属蛋白酶、纤维粘连蛋白1表达的影响观察
Effect of curcumin on expression of matrix metalloproteinase and fibronectin in human retinal pigment epithelium
摘要
Abstract
Objective To observe the effect of curcumin on the expression of matrix metalloproteinase(MMP)and fibronectin 1(FN1)in human retinal pigment epithelium(RPE).Methods Human RPE cells were divided into two groups:the curcumin group and control group;cells in the curcumin group were added with 0.5×10-5 mol/L curcumin,and cells in the control group were not treated with curcumin.After cells of the two groups were continuously incubated for 48 h,real-time fluorescence quantitative PCR was used to detect the matrix metalloproteinase(MMP)1,MMP2,MMP3,MMP9,and FN1 mRNAs,and Western blotting was used to detect the MMP1,MMP2,MMP3,MMP9,FN1 proteins in cells.Results The relative expression levels of MMP1,MMP2,MMP3,MMP9,and FN1 mRNAs in cells in the curcumin group were 0.41±0.14,5.020.22,1.090.21,0.470.28,and 2.500.21,and those in the control group were 5.100.38,6.430.15,0.270.19,1.820.12,and 6.830.18,respectively,with statistically significant differences be-tween these two groups(all P<0.05).The relative expression levels of MMP1,MMP2,MMP3,MMP9,and FN1 pro-teins were 6 155.33124.01,6 208.00157.33,8 126.33235.78,3 541.67 130.16,3 684.3390.22,respectively,in the curcumin group,versus 16 207.33210.19,8 053.3361.98,2 543.67122.20,9 477.33108.68,and 8 218.00239.86,respectively,in the control group,with statistically significant differences between these two groups(all P<0.05).Conclusion Curcumin up-regulates the expression of MMP1,MMP2,MMP9,FN1 and down-regulates the expression of MMP3 in human RPE cells.关键词
姜黄素/视网膜色素上皮细胞/基质金属蛋白酶/纤维粘连蛋白1/增殖性玻璃体视网膜病变/细胞外基质Key words
curcumin/retinal pigment epithelial cells/matrix metalloproteinase/fibronectin/proliferative vitreo-retinopathy/extracellular matrix分类
医药卫生引用本文复制引用
杜颖华,王淑然,滕达,梁小芳..姜黄素对人视网膜色素上皮细胞中基质金属蛋白酶、纤维粘连蛋白1表达的影响观察[J].山东医药,2024,64(21):35-38,4.基金项目
河北省重点研发计划项目(19277780D). （19277780D）
