饲料研究2024，Vol.47Issue(14)：75-80,6.DOI:10.13557/j.cnki.issn1002-2813.2024.14.013
外源谷胱甘肽对镉胁迫下4种紫花苜蓿光合作用和抗氧化酶活性的影响
Effects of exogenous glutathione on photosynthesis and antioxidant enzymes activity of four Medicago sativa species under cadmium stress
摘要
Abstract
The experiment aimed to explore the effects of adding exogenous glutathione on the photosynthesis and antioxidant enzyme activity of Medicago sativa in severely cadmium(Cd)polluted pasture areas.The materials for the experiment were Zhongmu No.l,WL525HQ,Gannong No.3,and Gannong No.9,with three groups set up:The control group(CK group,Hoagland culture solution),the Cd treatment group(Cd group,Hoagland+15 mg/L Cd),and the combined treatment group(Cd+GSH group,Hoagland+15 mg/L Cd+25 mg/L GSH)for hydroponic experiments.The relative water content of leaves(RWC),chlorophyll content(SPAD),stomatal conductance(Gs),net photosynthetic rate(Pn),intercellular CO2 concentration(Ci),transpiration rate(Tr),as well as the content of malondialdehyde(MDA),proline(Pro),and antioxidant enzyme activity were measured.The results showed that compared with the Cd group,the RWC,SPAD,Gs,Pn,Ci,and Tr of each variety in the Cd+GSH group were all improved to some extent.Compared with the Cd group,the MDA content of each variety in the Cd+GSH group was significantly reduced(P＜0.05),and the Pro content of WL525HQ,Gannong No.3,and Zhongmu No.1 was significantly reduced after the addition of exogenous GSH(P＜0.05).Compared with the Cd group,the activities of peroxidase(POD)and ascorbate peroxidase(APX)in each variety of the Cd+GSH group were significantly reduced(P＜0.05),the superoxide dismutase(SOD)activity of Gannong No.9 and Gannong No.3 was significantly reduced(P＜0.05),and the activity of catalase(CAT)in WL525HQ,Gannong No.9,and Gannong No.3 was significantly increased(P＜0.05).The study indicates that the addition of exogenous GSH can alleviate the toxic effects of Cd on Medicago sativa in severely Cd-polluted pasture areas,reduce oxidative damage,and improve its adaptability,and there is a certain correlation between various physiological indicators of alfalfa.关键词
镉胁迫/紫花苜蓿/谷胱甘肽/光合作用/抗氧化酶Key words
cadmium stress/Medicago sativa/glutathione/photosynthesis/antioxidant enzymes分类
农业科技引用本文复制引用
周德,黄薇,唐卿雁,李博,钱朋,杨姝..外源谷胱甘肽对镉胁迫下4种紫花苜蓿光合作用和抗氧化酶活性的影响[J].饲料研究,2024,47(14):75-80,6.基金项目
国家自然科学基金项目(项目编号:31860112) （项目编号:31860112）
