饲料研究2024，Vol.47Issue(14)：107-112,6.DOI:10.13557/j.cnki.issn1002-2813.2024.14.018
枯草芽孢杆菌BYA-KC-4产表面活性素发酵条件的优化
Optimizing fermentation conditions for production of surfactin from Bacillus subtilis BYA-KC-4
李高强 1张旭斌 1牛俊轲 1刘永立 1张锋 1高原源 1李梦洁 1贵文娟1
作者信息
- 1. 焦作市佰役安生物工程有限公司,河南焦作 454350||河南省饲用替抗工程技术研究中心,河南焦作 454350
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摘要
Abstract
The experiment aimed to optimize the fermentation conditions for the high-yielding surfactin-producing strain Bacillus subtilis BYA-KC-4 and to screen for the best conditions for surfactin production.The experiment determined the levels of factors affecting surfactin production through single-factor tests.The Box-Behnken central composite design was used to further optimize the fermentation condition parameters.The results showed that under the optimal fermentation conditions,namely a temperature of 33℃,an initial pH value of 6.8,an inoculum volume of 4.8％,and a rotation speed of 201 r/min,the yield of surfactin reached 560.25 mg/L.Compared with the 425.36 mg/L before optimization,it increased by 1.32 times.The study indicates that the experiment can provide a reference for surfactin production by Bacillus subtilis BYA-KC-4 and for industrial production.关键词
表面活性素/枯草芽孢杆菌/响应面优化/发酵条件Key words
surfactin/Bacillus subtilis/response surface optimization/fermentation conditions分类
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李高强,张旭斌,牛俊轲,刘永立,张锋,高原源,李梦洁,贵文娟..枯草芽孢杆菌BYA-KC-4产表面活性素发酵条件的优化[J].饲料研究,2024,47(14):107-112,6.
