饲料研究2024，Vol.47Issue(14)：134-140,7.DOI:10.13557/j.cnki.issn1002-2813.2024.14.023
基于Myco净化柱建立快速测定饲料中真菌毒素的UPLC-MS/MS法
Establishment of a rapid UPLC-MS/MS method for determination of mycotoxins in feed based on Myco purification column
摘要
Abstract
The experiment aims to establish a convenient and rapid universal quick purification column as a pre-treatment method,shortening the pre-treatment time by optimizing steps such as extraction and purification,and conducting rapid quantitative analysis of common mycotoxins in feed.The experiment has established an UPLC-MS/MS method for the rapid determination of seven mycotoxins in feed based on the Myco purification column filled with MOFS materials.The feed sample is extracted with acetonitrile-water,vortexed,loaded and purified through the Myco purification column,with 5 mmol/L ammonium formate-methanol as the mobile phase,and quantified by the external standard method using UPLC-MS/MS.The results show that the seven mycotoxins have a good linear relationship in the range of 0.5～500 μg/L,with a correlation coefficient(R2)＞0.99.The method detection limit is 0.5～2.0 μg/kg,and the quantification limit is 2.0～5.0 μg/kg.The recovery rates of the seven toxins at low,medium,and high levels of spiking in blank samples are 82.3％～96.8％,with relative standard deviations of 2.1％～9.3％.The results indicate that the UPLC-MS/MS method established based on the Myco purification column for the rapid determination of mycotoxins in feed is simple to handle and reliable,suitable for the quantitative determination of the seven mycotoxins in most feeds.关键词
UPLC-MS/MS/真菌毒素/饲料/快速测定Key words
UPLC-MS/MS/toxin/feed/rapid detection分类
农业科技引用本文复制引用
胡剑,郑百芹,谷守国,丛倩倩,裴小亮,刘宇奇,李梁,张建雄..基于Myco净化柱建立快速测定饲料中真菌毒素的UPLC-MS/MS法[J].饲料研究,2024,47(14):134-140,7.基金项目
河北省现代农业产业技术体系奶牛创新团队三期建设项目 （）
河北省第四批高端人才项目 （）
院士合作重点单位项目 （）
河北省现代农业产业技术体系建设专项资金(项目编号:HBCT2024280206) （项目编号:HBCT2024280206）
