山西医科大学学报2024，Vol.55Issue(7)：828-834,7.DOI:10.13753/j.issn.1007-6611.2024.07.003
厚朴酚对人乳腺癌MCF-7细胞增殖和程序性坏死的影响
Effect of magnolol on necroptosis of human breast cancer MCF-7 cells
摘要
Abstract
Objective To investigate the effect of magnolol on the cell proliferation and the necroptosis of human breast cancer cells and its possible mechanism.Methods Human breast cancer cell line MCF-7 cells were cultured in vitro.The MCF-7 cells were treated with 0,5,10,20,40,80 μmol/L magnolol for 24,48,72 h,or treated with 40 μmol/L magnolol with or without necroptosis inhibitor Nec-1(40 μmol/L)for 24 h,and then the cell viability was detected by MTT assay.The MCF-7 cells were treated with 0,2,4,8 μmol/L magnolol for 10 d,and then the colony-forming ability of MCF-7 cells was detected by colony-formation experiments.After the MCF-7 cells were treated with 40 µmol/L magnolol for 24 h,the morphological changes of MCF-7 cells were observed under transmission electron microscope.The MCF-7 cells were treated with 0,10,20,40,80 µmol/L magnolol for 24 h,and then the necrop-tosis and ROS level were detected by flow cytometry,and the protein expressions of RIP1,RIP3,p-RIP3,MLKL,and p-MLKL were detected by Western blot.Results Compared with 0 μmol/L magnolol,5,10,20,40,80 μmol/L magnolol significantly inhibited MCF-7 viability in a dose dependent manner(P＜0.01).The viability of MCF-7 cells was higher in magnolol+Nec-1 group than in magnolol group(P＜0.01).Compared with 0 µmol/L magnolol,2,4,8 μmol/L magnolol inhibited the cell colony formation.The formation of vacuoles and the vacuoles break were observed under transmission electron microscope after treatment with 40 µmol/L mag-nolol for 24 h.Compared with 0 µmol/L magnolol,the necroptosis rate,the ROS level and the protein levels of RIP1,RIP3,p-RIP3,MLKL and p-MLKL were significantly upregulated after treatment with 10,20,40,80 μmol/L magnolol(P＜0.01).Conclusion Magnolol can induce the necroptosis of human breast cancer MCF-7 cells,which is related to the activation of RIP3-MLKL signaling pathway.关键词
厚朴酚/乳腺癌/细胞增殖/程序性坏死/RIP3/ROSKey words
magnolol/human breast cancer/cell proliferation/necroptosis/RIP3/ROS分类
医药卫生引用本文复制引用
朱静,陈洁,李红梅,吴成柱..厚朴酚对人乳腺癌MCF-7细胞增殖和程序性坏死的影响[J].山西医科大学学报,2024,55(7):828-834,7.基金项目
蚌埠医学院512人才培育计划项目(By51202202) （By51202202）
蚌埠医学院研究生创新项目(Byycxz23022) （Byycxz23022）
