山西医科大学学报2024，Vol.55Issue(7)：908-915,8.DOI:10.13753/j.issn.1007-6611.2024.07.013
白藜芦醇纳米混悬剂的制备及体外初步评价
Preparation and preliminary evaluation in vitro of resveratrol nanosuspensions
摘要
Abstract
Objective To prepare and in vitro evaluate the resveratrol(RES)nanosuspension.Methods RES nanosuspensions were prepared by precipitation method.With the average particle size and the polydispersity index(PDI)as evaluation indexes,the orthogonal test was used to optimize the formulation process.The freeze-dried powder of nanosuspension was prepared by freeze-drying method and characterized.The in vitro release of the preparation was studied by dialysis bag method.The ARPE-19 cell injury model was estab-lished by high glucose stimulation,and the effect of RES nanosuspension on the cell viability was evaluated by CCK-8 method.Results The optimal prescription process of RES was as follows:the concentration of RES was 6 mg/mL,the mass ratio of two stabilizers(PVP K17∶HPMC)was 2∶1,and the mass ratio of RES and stabilizer was 1∶2,and 5％mannitol was freeze-dried protective agent.The RES nanosuspension was relatively regular granule,with a drug loading of 28.04％and a cumulative release of 91.27％within 36 h.Compared with RES,RES nanosuspension pretreatment significantly increased the viability of ARPE-19 cells damaged by high glucose.Conclusion RES nanosuspension could increase the release rate of RES in vitro,and could attenuate the damage of ARPE-19 cells by high glucose and improve the cell survival rate.关键词
白藜芦醇/纳米混悬剂/ARPE-19细胞/高糖致细胞损伤/体外累计释放率Key words
resveratrol/nanosuspension/ARPE-19 cells/high glucose-induced cell damage/in vitro cumulative release rate分类
医药卫生引用本文复制引用
李梦婷,褚琳,何宁,刘家佳,徐维平..白藜芦醇纳米混悬剂的制备及体外初步评价[J].山西医科大学学报,2024,55(7):908-915,8.基金项目
国家自然科学基金项目(52072360) （52072360）
