四白汤对乙醇诱导下小鼠胃溃疡的防治作用研究OACSTPCD
Research on the protective effect of Sibai Decoction on ethanol-induced gastric ulcers in mice
[目的]探讨四白汤方(SBT)对乙醇(EtOH)诱导下胃溃疡(GU)模型小鼠胃黏膜损伤的防治作用及其分子机制,为其临床治疗GU提供理论依据.[方法]体外实验将5组小鼠灌服高、中、低剂量SBT 7 d后取血分离血清,用于培养细胞建立血清学研究模型.含药血清的培养基培养人胃黏膜上皮细胞-1(GES-1),细胞进入G0期后加入0.8 mol/L EtOH处理4 h建立GES-1细胞损伤模型,采用细胞计数试剂盒-8(CCK8)法检测EtOH处理后的细胞的活力和SBT含药血清对细胞增殖的影响.体内实验将60只小鼠平均分为6组,SBT预处理后,EtOH诱导小鼠胃黏膜损伤模型.观察SBT对小鼠体质量和进食量的影响,苏木精-伊红(HE)染色检测各组胃黏膜病理学损伤状况,酶联免疫吸附测定(ELISA)法检测血清中炎性标志物炎症相关白细胞介素6(IL-6)、白细胞介素1β(IL-1β)、肿瘤坏死因子α(TNF-α)和白细胞介素10(IL-10)蛋白的表达水平,生化检测试剂盒检测胃黏膜丙二醛(MDA)、活性氧簇(ROS)、还原型谷胱甘肽过氧化物酶(GSH-Px)和一氧化氮(NO)的水平,蛋白质免疫印迹分析(Western blot)检测磷脂酰肌醇3激酶(PI3K)/蛋白激酶B(AKT)/核因子-κB(NF-κB)通路相关蛋白磷酸化磷脂酰肌醇3激酶(P-PI3K)、磷酸化蛋白激酶B(P-AKT)、磷酸化核因子κB抑制因子α(P-IκBα)、核内核因子κB p65(Nuclear NF-κB p65)、胞质核因子κB p65(Cytoplasm NF-κB p65)的表达.[结果]体外实验结果表明SBT能显著促进EtOH损伤的GES-1细胞增殖,对细胞无明显毒性,明显提高细胞在EtOH中的活力.体内实验结果表明SBT预处理后可显著改善EtOH诱导的GU模型小鼠的体质量及进食量(P<0.05);减轻病理组织学损伤;降低小鼠血清炎性标志物IL-1β、TNF-α和IL-6的表达(P<0.01),升高IL-10的表达(P<0.01);降低小鼠胃黏膜组织ROS的水平(P<0.01),升高GSH-Px的水平(P<0.01),对MDA及NO 影响则不显著;显著降低 P-PI3K/PI3K、P-AKT/AKT 和 Nuclear NF-κB p65/Cytoplasm NF-κB p65 的蛋白相对表达量(P<0.01),显著提高P-IκBα/IκBα的蛋白相对表达量(P<0.01).[结论]SBT通过提高抗氧化应激能力、减轻炎症反应、调节P13K/AKT/NF-κB通路,对EtOH诱导的小鼠GU具有显著的防治作用,为进一步开发和利用SBT治疗GU提供了理论依据.
[Objective]This study aims to investigate the prophylactic and therapeutic effects of Sibai Decoction(SBT)on gastric ulcers(GU)induced by ethanol(EtOH)in mice,and to explore its underlying molecular mechanisms,thus providing a theoretical basis for its clinical application in treating GU.[Methods]In vitro,five groups of mice were administered with high,medium,and low doses of SBT for 7 days,after which blood was collected to separate serum for the establishment of a serological research model.Human gastric epithelial cells(GES-1)were cultured in media containing drug-serum and treated with 0.8 mol/L EtOH for 4 hours after entering the G0 phase to establish a GES-1 cell injury model.Cell viability and the effects of SBT-containing serum on cell proliferation were measured using the Cell Counting Kit-8(CCK8)method.In vivo,60 mice were divided into six groups evenly and pre-treated with SBT before EtOH induction to develop a gastric mucosal injury model.The effects of SBT on the mouse weight and food intake were observed.Histopathological changes in the gastric mucosa were assessed by Hematoxylin and Eosin(HE)staining.Levels of inflammatory markers,including Interleukin 6(IL-6),Interleukin 1 Beta(IL-1 β),Tumor Necrosis Factor Alpha(TNF-α),and Interleukin 10(IL-10)protein expression levels,were measured in the serum using Enzyme-Linked Immunosorbent Assay(ELISA).Gastric mucosal levels of Malondialdehyde(MDA),Reactive Oxygen Species(ROS),Glutathione Peroxidase(GSH-Px),and Nitric Oxide(NO)were assessed using biochemical kits.The expression of proteins involved in the PI3K/AKT/NF-κB pathway,including Phosphorylated Phosphatidylinositol 3-Kinase(P-PI3K),Phosphorylated Protein Kinase B(P-AKT),Phosphorylated Nuclear factor kappa-B inhibitor alpha(P-IκBα),Nuclear Nuclear Factor Kappa B p65(Nuclear NF-κB p65),and Cytoplasmic Nuclear Factor Kappa B p65(Cytoplasm NF-κB p65),were analyzed by Western blot.[Results]In vitro experiments demonstrated that SBT significantly promoted the proliferation of EtOH-damaged GES-1 cells,with no apparent toxicity,and notably improved cell viability in the presence of EtOH.In vivo results indicated that SBT pre-treatment significantly ameliorated the body weight(P<0.05)and food intake(P<0.05)in the EtOH-induced GU model in mice;reduced histopathological damage;decreased serum levels of inflammatory markers IL-iβ(P<0.01),TNF-α(P<0.01)and IL-6(P<0.01),and increased IL-10 expression(P<0.01);lowered ROS levels(P<0.01)and increased GSH-Px levels(P<0.01)in the gastric mucosa,while effects on MDA and NO were not significant;significantly decreased the relative protein expression of P-P13K/PI3K(P<0.01),P-AKT/AKT(P<0.01)and Nuclear NF-κB p65/Cytoplasm NF-κB p65(P<0.01),and significantly increased P-IκBα/IκBαexpression(P<0.01).[Conclusion]SBT exhibits significant prophylactic and therapeutic effects on EtOH-induced GU in mice by enhancing antioxidative stress capacity,mitigating inflammatory responses,and regulating the PI3K/AKT/NF-κB pathway,providing a theoretical foundation for the further development and application of SBT in treating GU.
李平香;高瑞阳;李景尧;郑艳
青岛大学医学部中西医结合学科,青岛 266023青岛市中医医院(市海慈医院)青岛大学附属青岛市海慈医院,青岛 266033
中医学
四白汤乙醇胃溃疡抗氧化应激抗炎
Sibai Decoctionethanolgastric ulcerantioxidant stressanti-inflammatory
《天津中医药》 2024 (008)
1030-1038 / 9
青岛市2015-2016年度中医药研究计划(2015-zyy013).
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