滨海盐碱地苜蓿-小黑麦轮作对土壤细菌和真菌群落多样性与网络结构的影响OA北大核心CSTPCD

Incorporating legumes into crop rotations not only promotes sustainable ecosystem development,but also provides a key way to increase crop yields,especially in low-input and low-diversity agricultural systems.However,the effects of legume inclusion on the soil microbial communities remain unclear.In this study,the responses of soil bacterial and fungal community structures and co-occurrence network patterns in winter triticale-summer maize(MT)and alfalfa-winter triticale(AT)rotations were investigated using high-throughput sequencing technology in a typical coastal saline-alkali land.The results showed that AT had little effect on bacterial community diversity but decreased fungal richness due to soil nitrogen and phosphorus changes.Non-metric multidimensional scaling(NMDS)analysis showed that AT altered the fungal community structure,but not the bacterial community structure,which was associated with pH and soil organic carbon differences.Network analysis showed that AT increased the complexity and stability of the bacterial network structure,including an increase in nodes and links,average degree,keystone nodes,and negative links as well as a decrease in average path distance,but decreased the complexity and stability of the fungal network structure.This highlights that the contrasting responses of the bacterial and fungal communities to legume inclusion should be considered when exploring the effects of legume-based rotation on the soil microbial community.