代谢矿井CH4和CO气体的微生物提取及生长代谢特性OA北大核心CSTPCD

In order to reduce the production and accumulation of combustible gases in goaf such as CH4 and CO and so on,restrain the explosion of combustible gases and ensure the safety of coal mining,the bioremediation method was adopted to enrich and acclimate the microorganisms in different environ-mental samples of the mines,the growth characteristics and community structure of bacteria after en-richment were analyzed by high-throughput techniques,and the growth metabolic characteristics of CH4 oxidizing bacteria and CO oxidizing bacteria were mastered after isolation and purification.The results show that the sole carbon source method can significantly improve the enrichment degree of target mi-croorganisms,and the dominant bacteria in different samples are different,the CH4 metabolic rate rea-ches 80%,and the CO metabolic rate exceeds 95%after enrichment.After purification,2 strains of CH4 oxidizing bacteria(S2,T7)and 3 strains of CO oxidizing bacteria(M1,M7,M13)are extracted,and the growth cycle of the microorganisms is determined.Among them,CO gas is basically metabolized by CO oxidizing bacteria in the logarithmic period,with the CO metabolic rate exceeding 95%,CH4 gas metabolism accompanied the whole growth process of CH4 oxidizing bacteria,and its metabolic process lagged behind that of the CO oxidizing bacteria,the CH4 metabolic rates of S2 and T7 are 76.1%and 74.5%,respectively.In addition,CH4 oxidizing bacteria uses more CH4 gas for growth and reproduc-tion,showing higher growth concentration of bacterial fluid,higher O2 consumption and lower CO2 pro-duction than CO oxidizing bacteria,but CO oxidizing bacteria has stronger gas metabolic activity than CH4 oxidizing bacteria.The research results can provide a basis for the comprehensive prevention and control of the combustible gases in goaf such as CH4 and CO,and it is of great significance to prevent the thermal power disasters caused by spontaneous combustion in goaf and to ensure the safety and green mining of coal mines.