现代医药卫生2024，Vol.40Issue(16)：2735-2739,5.DOI:10.3969/j.issn.1009-5519.2024.16.008
2018-2019年上海市普陀区16起聚集性呕吐腹泻疫情中诺如病毒的流行与分析
Prevalence and analysis of norovirus in 16 clusters of vomiting and diarrhea outbreaks in Putuo District of Shanghai from 2018 to 2019
摘要
Abstract
Objective To understand the genotyping and epidemiological characteristics of norovirus in 16 clusters of vomiting and diarrhea in Putuo District of Shanghai,and to provide technical support and data reference for the treatment and prevention of clusters of vomiting and diarrhea caused by norovirus in the dis-trict and the whole city.Methods A total of 136 anal swab samples were collected from people involved in 16 clusters of vomiting and diarrhea outbreaks in Putuo District of Shanghai from 2018 to 2019.The nucleic acid of norovirus was detected by real-time fluorescence polymerase chain reaction.The polymerase-capsid protein link region was amplified by real-time fluorescence polymerase chain reaction and sequenced in the positive samples of typical cases.Genotyping and phylogenetic tree were constructed by bioinformatics tools.Results Among the 136 specimens,62(45.59%)were positive for norovirus GⅡ nucleic acid,and zero was positive for norovirus GI nucleic acid.42 noroviruses were successfully sequenced,and four genotypes were obtained by sequence align-ment analysis.Among the 16 clusters of vomiting and diarrhea outbreaks,nine were GⅡ.P16-GⅡ.2(56.25%),two were GⅡ.P7-GⅡ.6(12.50%),two were GⅡ.P12-GⅡ.3(12.50%),and three were GⅡ.Pe-GⅡ.4(18.75%).Conclusion Norovirus is an important pathogen causing clustered vomiting and di-arrhea in Putuo District of Shanghai from 2018 to 2019.It is mainly GⅡ group and has a variety of genotypes,among which GⅡ.P16-GⅡ.2 is the main epidemic strain.关键词
诺如病毒/聚集性呕吐腹泻疫情/基因分型/流行分析Key words
Norovirus/Cluster vomiting and diarrhea outbreaks/Genotyping/Prevalence analysis分类
医药卫生引用本文复制引用
闫浩,顾文超,吴婷婷,李勇,张玉,唐海丰..2018-2019年上海市普陀区16起聚集性呕吐腹泻疫情中诺如病毒的流行与分析[J].现代医药卫生,2024,40(16):2735-2739,5.基金项目
上海市加强公共卫生体系建设三年行动计划项目(GWVI-3). （GWVI-3）
