中国动物检疫2024，Vol.41Issue(8)：112-120,9.DOI:10.3969/j.issn.1005-944X.2024.08.020
羊败血性链球菌病灭活疫苗效力检验小鼠免疫攻毒法研究
Research on the Efficacy Test of Inactivated Vaccines against Sheep Septicemic Streptococcal Disease Using Mouse Immunochallenge
摘要
Abstract
In order to solve the difficulties in animal screening,unstable animal quality,high economic costs and other problems in the efficacy test of inactivated vaccines against sheep septicemic streptococcal disease using target animal immunochallenge,a research on the alternative methods of immunochallenge in experimental animals was conducted.Based on target animal immunochallenge,the minimum protective dose and non-protective critical dose of the vaccine were determined,and a parallel research was conducted after confirming the variety of mice to select the optimal dose that could reflect the difference of the vaccine in the mouse model.The results showed that,for qualified vaccines,1/2 dilution was the minimum immune dose,and 1/4 was the critical dose in noncompliance of regulations;KM mice were the most suitable alternatives;2MLD mixture of bacterial solution was used as the optimal dose to challenge the immune group and control group,all the mice in the control group died,and at least 70%of those in the immune group were protected.An alternative method of efficacy test of inactivated vaccines against sheep septicemic streptococcal disease using mouse immunochallenge was established by the study,which will improve the stability and reliability of the efficacy test of such vaccines,and support the prevention and control of sheep septicemic streptococcal disease.关键词
羊败血性链球菌病/马链球菌兽疫亚种/疫苗效力检验/免疫攻毒法/平行关系/替代方法Key words
sheep septicemic streptococcal disease/Streptococcus equi subsp zooepidemicus/vaccine efficacy test/immunochallenge/parallel relationship/alternative method分类
畜牧业引用本文复制引用
张一帜,刘燕,任小侠,冯妍,李建,辛凌翔,孙淼,王豪杰,佟仁冬,朱良全..羊败血性链球菌病灭活疫苗效力检验小鼠免疫攻毒法研究[J].中国动物检疫,2024,41(8):112-120,9.基金项目
中国兽医药品监察所兽药行业公益性重点专项(GY202103) （GY202103）
