饲料中添加南极磷虾粉对中华绒螯蟹繁殖性能及胚胎质量的影响OA北大核心CSTPCD

To explore the nutrient enrichment effect of Antarctic krill meal to Eriocheir sinensis brood stocks,a 60-d feeding trial was conducted on the reproductive performance and embryo quality of the Chinese mitten crab,Eriocheir sinensis.Three diets were formulated,incorporating varying levels of Antarctic krill meal at 0％,10％and 20％,with a control group fed razor clam Sinonovacula constricta.The hepatopancreatic weight(HW),hepatopancreas index(HSI),gonadal weight(GW),and gonadosomatic index(GSI)were detected after 60 d of nutritional enrichment.Each treatment had three replicate tanks stocked with five males and 10 females.The reproductive performance of broodstock and embryo quality—such as egg production,fecundity,reproductive index,egg diameter,weight,and biochemical and fatty acid compositions—were analyzed.The results showed:(1)The HW and HSI of female crabs in each group significantly decreased(P＜0.05),while GW and GSI increased significantly(P＜0.05)after 60 d,with the 20％group showing the highest values.(2)Egg production and fecundity of female crabs reached their peak in the 20％group,with no significant differences(P＞0.05)in reproductive indices among all groups.(3)The phospholipid content in the 20％group was significantly higher than that in the other groups(P＜0.05),the highest triglyceride(TG)content was found in the 10％group,with an average of(1449.73±101.38)μg/g,significantly higher than other groups(P＜0.05),cholesterol content in the 0％and control groups were significantly higher than that in the 10％and 20％groups(P＜0.05).No significant differences were observed in egg diameter,egg weight,moisture,crude protein,and crude fat between the groups(P＞0.05).(4)The content of C20:2 and C20:4n6 was highest in the 0％group,with a significant difference compared to the 10％group(P＜0.05).However,no significant differences were found in the total content of saturated fatty acids(SFA),mono-unsaturated fatty acids(MUFA)and poly unsaturated fatty acids(PUFA)among all groups(P＞0.05).The results indicate that 10％～20％and 20％dietary Antarctic krill meal could promote gonadal development of the female E.sinensis and significantly improve reproductive performance and embryo quality,respectively.