中国动物传染病学报2024，Vol.32Issue(4)：11-16,6.
IL-12在RSV感染小鼠气道重塑中的作用机制分析
Action Mechanism of Il-12 in Airway Remodeling in Mice with RSV Infection
摘要
Abstract
To explore the action mechanism of interleukin 12(IL-12)in airway remodeling in mice with Respiratory syncytial virus(RSV)infection,the type Ⅱ alveolar epithelial cells(AEC Ⅱ)of rats were cultured in vitro and the release rules of IL-12 in the cells were observed after RSV inoculation.The RSV infection model was developed in mice with IL-12 P40 gene knockout[IL-12 P40(-/-)]by nasal drip method.The infected mice were examined for pathological changes of lung tissues,Th1/Th2 cytokine levels in bronchoalveolar lavage fluid(BALF),signal transducer and activator of transcription 4(STAT4)and airway remodeling markers in lung tissues.At 3,12 and 24 h after infection,IL-12 level in infection group was lower than that in control group(P<0.05).The lymphocytes,neutrophils and eosinophils,IL-5,IL-13,pathological scores of airway inflammation,and expression levels of ColI,FN-1 and α-SMA in mice with IL-12 P40(-/-)were higher than those in control mice while macrophages,levels of IL-12,IFN-γ and p-STAT4/STAT4 were lower than those in control mice(P<0.05).These results showed that RSV infection induced the down-regulation of IL-12 level in AEC Ⅱ and control mice.IL-12 deficiency would aggravate airway inflammation and airway remodeling in RSV mice,which might be related to inhibiting STAT4 activation.关键词
呼吸道合胞病毒/白细胞介素12/气道炎症/转录激活因子4/气道重塑Key words
Respiratory syncytial virus/interleukin 12/airway inflammation/signal transducer and activator of transcription 4/airway remodeling分类
医药卫生引用本文复制引用
陶双,董善武..IL-12在RSV感染小鼠气道重塑中的作用机制分析[J].中国动物传染病学报,2024,32(4):11-16,6.基金项目
湖北省卫生健康委员会2019～2020年度面上项目(WJ2019M020) （WJ2019M020）
