中国动物传染病学报2024，Vol.32Issue(4)：39-45,7.
一株H9N2亚型禽流感病毒抗原变异株产生原因的分析
Analysis of the Antigen Variation of A H9N2 Avian Influenza Virus Strain
摘要
Abstract
At present,the H9N2 subtype avian influenza virus is the most important low pathogenic variant in China,causing serious economic losses to the poultry industry.The inactivated H9N2 avian influenza virus vaccine is the most effective way to prevent and control the disease.However,the H9N2 virus is prone to antigenic variation under immune pressure,resulting in the failure of vaccine immunity.The mechanism is still unclear.In the previous study,we screened escape strains carrying a single point mutation of HAD201N by simulating the immune stress of H9N2 avian influenza but the reason why HAD201N led to the emergence of H9N2 virus escape strains was still unclear.In the present study,we rescued a strain of A/Chicken/Shanghai/1/2006(H9N2)(rSH1)and its immune escape strain rSH1-HAD201N by using the 8-plasmid reverse genetic technology.In order to identify whether HAD201N caused immune escape of the virus by affecting the replication ability of H9N2 avian influenza virus,MDCK cells were inoculated with the rSH1 virus at 0.001 MOI and its growth curve was measured.The results showed that the point mutation of HAD201N significantly reduced the replication ability of rSH1 virus but the change of virus replication ability was not the main reason for the escape of rSH1 virus.In order to investigate whether HAD201N produced virus escape strains by affecting the antigenic variation of H9N2 virus,the original virus and mutant virus were emulsified with adjuvant to immunize chickens for preparation of antiserum.Cross-HI test was used to calculate the R value and analyze the antigenic variation.The R value of rSH1-HAD201N was 2,indicating that HAD201N caused significant antigenic variation in rSH1,allowing the virus to escape immune stress.This study laid a foundation for further exploring the antigenic variation of H9N2 avian influenza virus and vaccine development.关键词
H9N2亚型禽流感病毒/反向遗传技术/点突变/免疫逃逸Key words
H9N2 subtype avian influenza virus/reverse genetics technology/point mutation/immune escape分类
农业科技引用本文复制引用
乔淑媛,滕巧泱,李雪松,刘芹防,张志飞,王思琪,李泽君,杨健美,王彩云..一株H9N2亚型禽流感病毒抗原变异株产生原因的分析[J].中国动物传染病学报,2024,32(4):39-45,7.基金项目
中国农业科学院创新工程基金(CAAS-ASTIP-SHVRI-2014-4) （CAAS-ASTIP-SHVRI-2014-4）
国家青年科学基金项目(31700136) （31700136）
上海市科技兴农项目(2021-02-08-00-12-F00746) （2021-02-08-00-12-F00746）
中央级公益性科研院所基本科研业务费专项(JB03) （JB03）
