中国动物传染病学报2024，Vol.32Issue(4)：46-52,7.
一株羊口疮病毒的全基因组测序及功能分析
Whole Genome Sequencing and Functional Analysis of Orf Virus
李阳 1马园 1王国华 2田普厚 2李成龙 2张七斤1
作者信息
- 1. 内蒙古农业大学兽医学院,呼和浩特 010018
- 2. 内蒙古必威安泰生物科技有限公司,呼和浩特 011500
- 折叠
摘要
Abstract
In order to better understand the database information and functional analysis of the Orf virus(ORFV)genome,this experiment used the Illumina TruSeq™ Nano DNA Sample Prep Kit method to construct a library to perform whole genome sequencing of the isolate ORFV-Q,and NR,GO and KEGG annotations etc.for bioinformatics analysis.The test results showed the genome size of 127 160 bp,abnormal distribution of GC content and prediction of 123 genes.In addition,16 genes were annotated according to the NR database annotation comparison.The GO database annotation revealed 30 genes involved in biological pathways,33 cellular component genes and 8 molecular function genes.By alignment and comparison with the KEGG metabolic pathway database,there were 5 genes that corresponded.The Swiss-Prot database annotation discovered a total of 75 genes whose protein sequence functions were annotated.This study not only provided data support for ORFV genome research but also laid a theoretical basis for subsequent vaccine development,genome component analysis and gene function annotation.关键词
羊口疮病毒/全基因组测序/功能分析Key words
Orf virus/whole genome sequencing/functional analysis分类
农业科技引用本文复制引用
李阳,马园,王国华,田普厚,李成龙,张七斤..一株羊口疮病毒的全基因组测序及功能分析[J].中国动物传染病学报,2024,32(4):46-52,7.
