中国动物传染病学报2024，Vol.32Issue(4)：53-59,7.
猪链球菌重组磷酸ABC转运体ATP酶的免疫保护效果评价
Evaluation of Immunoprotection of Recombinant Phosphate ABC Transporter ATPase of Streptococcus suis in Mice
摘要
Abstract
Streptococcus suis(S.suis)is one of the most important zoonotic pathogens and brings great losses to the pig industry.The objective of the present study was to evaluate the immunoprotective effect of the recombinant phosphate ABC transporter ATPase of S.suis in mice.The vector for the phosphate ABC transporter ATPase of S.suis was constructed and the recombinant ATPase protein was expressed via prokaryotic expression.The resulting recombinant ATPase protein was used to prepare a subunit vaccine for mouse immunization.The ELISA titers of serum antibodies,immunoprotection rate,bacterial loads in different organs and inflammatory factors were examined at two weeks after a booster immunization.The results showed that the recombinant protein mainly existed in supernatant of E.coli culture.The recombinant protein induced a high level of IgG antibodies and 60%immunoprotection against S.suis serotype 2 infection in mice.In addition,bacterial loads were significantly reduced in brains,hearts and lungs of the immunized mice and the mRNA expression levels of IL-1β,IFN-γ,IL-6,IL-8 and TNF-α were also significantly up-regulated.The results from this study indicated that recombinant phosphate ABC transporter ATPase was a potential subunit vaccine candidate for prevention of S.suis serotype 2 infection.关键词
猪链球菌/亚单位疫苗/磷酸ABC转运体ATP酶/免疫保护Key words
Streptococcus suis/subunit vaccine/phosphate ABC transporter ATPase/immunoprotection分类
农业科技引用本文复制引用
张小玲,李日顺,樊擎莹,王海堃,王瑜欣,汪洋..猪链球菌重组磷酸ABC转运体ATP酶的免疫保护效果评价[J].中国动物传染病学报,2024,32(4):53-59,7.基金项目
国家自然科学基金项目(321728520 （）
河南省杰出青年科学基金项目(222300420005) （222300420005）
河南省高校科技创新团队(24IRTSTHN033) （24IRTSTHN033）
