中国动物传染病学报2024，Vol.32Issue(4)：60-66,7.
PCV-2、PCV-3、PCV-4三重PCR鉴别检测方法的建立与应用
Development and Application of Triple PCR for Detection and Differentiation of PCV-2,PCV-3 and PCV-4
摘要
Abstract
To develop a rapid method for detection and differentiation of PCV-2,PCV-3 and PCV-4,the specific primers of PCV-2,PCV-3 and PCV-4 were designed and synthesized,for a triple PCR method.The specific bands of 216,415,678,216 bp/415 bp/678 bp were amplified from PCV-2,PCV-3,PCV-4 and PCV-2/PCV-3/PCV-4.The triple PCR method did not amplify PRV,PPV,PRRSV,CSFV,PEDV and TGEV.The minimum detection limits were 1.0×102 copies/µL for PCV-2,1.0×103 copies/µL for PCV-3 and 1.0×103 copies/µL for PCV-4.The coincidence rates of the single PCR method for PCV-2,PCV-3 and PCV-4 were all 100%.Then,85 clinical samples were tested by this method and the positive rates of PCV-2,PCV-3 and PCV-4 were 21.17%,14.12%and 4.71%,respectively.These results showed that the developed triple PCR method for differential detection of PCV-2,PCV-3 and PCV-4 had good specificity,sensitivity,accuracy and clinical applicability,thus provided a simple and rapid molecular biological diagnostic technology for PCV-2,PCV-3 and PCV-4.关键词
PCV-2/PCV-3/PCV-4/三重PCR/鉴别检测Key words
PCV-2/PCV-3/PCV-4/triple PCR/detection分类
农业科技引用本文复制引用
王勤,欧云文,汪洋,潘琴,刘俐君,何博..PCV-2、PCV-3、PCV-4三重PCR鉴别检测方法的建立与应用[J].中国动物传染病学报,2024,32(4):60-66,7.基金项目
四川省科技计划项目(2020JDRC0146) （2020JDRC0146）
甘肃省科技计划项目(20YF3NA005) （20YF3NA005）
