中国动物传染病学报2024，Vol.32Issue(4)：97-103,7.
猪流行性腹泻病毒CH/HB/CZ01的分离鉴定及致病性研究
Isolation,Identification and Pathogenicity of Porcine Epidemic Diarrhea Virus CH/HB/CZ01
摘要
Abstract
In order to obtain local isolates of Porcine epidemic diarrhea virus(PEDV)for preliminary understanding of their biological characteristics and pathogenicity,virus isolation and identification were performed in in Hebei province.The isolated PEDV was analyzed for its genetic evolution and pathogenicity in piglets.The samples collected in this area were inoculated onto Vero cells for continuous blind passages and the PEDV CH/HB/CZ01 strain was isolated by RT-PCR detection and gene sequencing identification.The genetic evolution analysis of the S gene of this strain was carried out using the sequences of the PEDV references published in GenBank.The results showed that the sequence homology between this strain and the popular strains found in recent years in China was 97.1%-98.3%,which was significantly higher than that of CV777,LZC,DR13 and other traditional strains and thus this strain belonged to the GⅡb subgroup.The isolated C H/HB/CZ01 virus culture was inoculated into healthy piglets with 2.0 mL each.,The inoculated piglets developed clinical signs such as diarrhea and their rectal swabs were tested PEDV positive by RT-PCR at 2 days post inoculation.The results showed that the isolated CH/HB/CZ01 virus was a mutant with strong pathogenicity,which laid a preliminary foundation for the study of the genetic and biological characteristics of the new PEDV strains in Hebei province.关键词
猪流行性腹泻病毒/遗传进化/S基因/致病性Key words
Porcine epidemic diarrhea virus/genetic evolution/S gene/pathogenicity分类
农业科技引用本文复制引用
翟刚,刘莹,赵云环,刘濮毓,王传文,李妍,范京惠,左玉柱..猪流行性腹泻病毒CH/HB/CZ01的分离鉴定及致病性研究[J].中国动物传染病学报,2024,32(4):97-103,7.基金项目
河北省农业产业技术体系生猪创新团队(HBCT2018110207) （HBCT2018110207）
