中国动物传染病学报2024，Vol.32Issue(4)：111-117,7.
银狐源沙门菌的分离鉴定、致病性及耐药性检测
Isolation,Identification,Pathogenicity and Drug Resistance of Salmonella from Diarrhea Silver Foxes
摘要
Abstract
In order to understand the pathogenicity,drug resistance and genotypes of Salmonella,194 anal swabs,feces and other disease tissues were collected from diarrhea silver foxes on different farms from 2019 to 2021 in Qinhuangdao area for bacterial isolation as well as CDV,CPV and Rotavirus testing by RT-PCR and PCR.The isolated Salmonella was identified PCR.The pathogenicity,drug resistance and genotypes of Salmonella were studied via experimental infection in mice,K-B drug sensitivity paper method and PCR.The results showed that CDV,CPV and Rotavirus were negative and 101 Salmonella strains were isolated from these samples,of which 78 Salmonella strains were pathogenic to mice with the mortality of 40%-100%.The resistance rates of these pathogenic Salmonella strains were more than 50%to ampicillin,amoxicillin and oxytetracycline and 5.1%-32.1%to other drugs.More than 48.7%of drug resistance genes were Sul1,Sul2,Sul3,aac(3)-Ⅰv,aac(6')-Ⅰb,TetA,TetM and TetR detected in and 7.7%-21.8%were other genes.Except for polymyxin drugs,there was a correlation between drug resistance phenotype and genotypes.This study provided a reference for the prevention and control of Salmonella infection in silver foxes in this area.关键词
银狐/腹泻/沙门菌/致病性/耐药性/耐药基因Key words
Silver fox/diarrhea/Salmonella/pathogenicity/drug resistance/drug resistance gene分类
农业科技引用本文复制引用
张召兴,韩平,李涵,张艳英,史秋梅..银狐源沙门菌的分离鉴定、致病性及耐药性检测[J].中国动物传染病学报,2024,32(4):111-117,7.基金项目
河北省现代农业产业技术体系建设专项资金(HBCT2024280205) （HBCT2024280205）
河北省创新能力提升计划项目(20567621H) （20567621H）
河北省第三批巨人计划中草药防治畜禽水产养殖动物传染病科研创新团队项目 （）
