中国动物传染病学报2024，Vol.32Issue(4)：118-124,7.
犬实验性感染乙脑病毒的研究
Experimental Infection of Japanese Encephalitis Virus in Dogs
摘要
Abstract
To investigate the immune response and clinical signs of Japanese encephalitis virus(JEV).3-month-old Beagle dogs were experimentally infected with a virulent JEV GZ strain from Guizhou and an attenuated SA14-14-2 strain.The serum samples were examined for changes of immunoglobulin M(IgM),immunoglobulin G(IgG),tumor necrosis factor-α(TNF-α),interferon-γ(IFN-γ)levels and T lymphocyte subsets CD4+/CD8+T lymphocytes cells.Dogs did not show clinical signs post infection with the virulent and attenuated JEV strains.The serum levels of IgM,IgG,TNF-α and IFN-γ and CD4+/CD8+ratio increased.No virus was detected in blood samples by RT-PCR and also no obvious lesions were found in tissue sections.In summary,dogs infected with the virulent and attenuated JEV strains produced immune responses without clinical signs and viremia.Therefore,as companion animals live in close proximity to humans,dogs were very suitable as sentinel animals for assessing the risk of JEV infection.关键词
乙脑病毒/免疫应答/哨兵动物/犬Key words
Japanese encephalitis virus/immune response/sentinel animals/dog分类
畜牧业引用本文复制引用
林泠,刘巧玲,禹光美,郑如雯,李芳芳,汤德元,黄涛..犬实验性感染乙脑病毒的研究[J].中国动物传染病学报,2024,32(4):118-124,7.基金项目
青年教师国家自然科学基金培育项目建议资助项目(黔科合平台人才[2017]5788-70) （黔科合平台人才[2017]5788-70）
贵州大学引进人才科研项目(贵大人基合字[2015]33) （贵大人基合字[2015]33）
贵州省科技计划项目(黔科合支撑[2018]2265) （黔科合支撑[2018]2265）
贵州省2016科学技术基金(黔科合基础[2016]1048) （黔科合基础[2016]1048）
