中国动物传染病学报2024，Vol.32Issue(4)：167-172,6.
牛病毒性腹泻病毒NS5B蛋白的截短表达及多克隆抗体制备
Prokaryotic Expression of Truncated BVDV NS5B Protein and Preparation of Polyclonal Antibodies
摘要
Abstract
The purpose of this study was to clone the gene fragment of the non-structural protein NS5B of Bovine viral diarrhea virus(BVDV)for prokaryotic expression of the truncated protein and prepare its polyclonal antibodies.The constructed plasmid pET-28a-NS5B was transformed into BL21 competent cells.The truncated NS5B protein was expressed with induction of IPTG and mainly released as inclusion bodies.The polyclonal antibodies prepared by immunizing mice had good reactivity and specifically recognized the eukaryotic expressed NS5B protein.The expression of truncated NS5B protein and preparation of polyclonal antibodies in this study laid the foundation for further studying the biological function of NS5B protein and developing a serological method for differential diagnosis of BVDV.关键词
牛病毒性腹泻病毒/NS5B蛋白/原核表达/多克隆抗体Key words
Bovine viral diarrhea virus/NS5B protein/prokaryotic expression/polyclonal antibody分类
农业科技引用本文复制引用
秦春雪,夏国建,何成强..牛病毒性腹泻病毒NS5B蛋白的截短表达及多克隆抗体制备[J].中国动物传染病学报,2024,32(4):167-172,6.基金项目
山东省重点研发项目(2017GNC10125,2019GSF107020) （2017GNC10125,2019GSF107020）
