中国动物传染病学报2024，Vol.32Issue(4)：195-200,6.
猪繁殖与呼吸综合征病毒GP5蛋白在昆虫杆状病毒表达系统中的表达与鉴定
Expression and Characterization of Porcine Reproductive and Respiratory Syndrome Virus GP5 Protein in Insect Baculovirus Expression System
摘要
Abstract
Porcine reproductive and respiratory syndrome virus(PRRSV)GP5 protein is the main envelope structural protein of the virus and main target protein of neutralizing antibodies,which is widely used for the development of new vaccines and diagnostic approaches for PRRS.In order to maintain the natural structure of the PRRSV GP5 protein,the PRRSV ORF5 gene was optimized according to the codon preference of Spdopterafrugiperda,subsequently subcloned into the baculovirus vector and transfected into the Sf9 insect cells for expression of the recombinant rBac-PRRSV-ORF5.Western blot analysis showed that the GP5 protein was efficiently expressed in the Sf9 insect cells and exhibited high immunogenicity.The recombinant GP5 protein was further purified with nickel ion affinity chromatography.Our data laid a foundation for the biological study and development of diagnostic methods for PRRSV.关键词
猪繁殖与呼吸综合征病毒/ORF5基因/GP5蛋白/表达/蛋白纯化Key words
Porcine reproductive and respiratory syndrome virus/ORF5 gene/GP5 protein/expression/protein purification分类
农业科技引用本文复制引用
黄袁慧,李义平,王传彬,马静,王乃迪,张昕,张莹,兰冰洁,刘玉良,倪建强,周智..猪繁殖与呼吸综合征病毒GP5蛋白在昆虫杆状病毒表达系统中的表达与鉴定[J].中国动物传染病学报,2024,32(4):195-200,6.基金项目
现代农业产业体系北京市创新团队(BAIC02-2021) （BAIC02-2021）
