栝楼桂枝颗粒对脑缺血再灌注损伤大鼠神经功能及突触可塑性的影响机制OA北大核心CSTPCD

OBJECTIVE To investigate the effect mechanism of Gualou guizhi granule on neurological function and synaptic plasticity in rats with cerebral ischemia-reperfusion injury.METHODS The rats were randomly divided into sham operation group,model group and Gualou guizhi granule group(3.6 g/kg),with 6 rats in each group.The cerebral ischemia-reperfusion injury model was established by the suture occlusion method.Two hours after modeling,the model rats were given relevant medicine or normal saline intragastrically,once a day,for 7 consecutive days.After the last medication,the neurological function of rats was evaluated[calculated by modified neurological severity scores(mNSS)and corner turning percentage];the neuronal apoptosis rate of brain histiocyte in the ischemic side of rats was detected in each group;the positive expressions of growth associated protein 43(GAP43)and microtubule associated protein 2(MAP2)were detected;protein expressions of neuron-specific nuclear protein(NeuN),synaptophysin-1(Syn-1),postsynaptic density protein-95(PSD-95),peroxisome proliferators-activated receptor γ(PPARγ),brain-derived neurotrophic factor(BDNF)and tropomyosin receptor kinase B(TrkB),as well as mRNA expressions of NeuN,Syn-1 and PSD-95 were detected.RESULTS Compared with the model group,mNSS,corner turning percentage and neuronal apoptotic rate were decreased significantly in Gualou guizhi granule group(P＜0.01);GAP43 represented weak immunoreactivity,and MAP2 represented moderate immunoreactivity;protein expressions of NeuN,Syn-1,PSD-95,PPARγ,BDNF,TrkB and mRNA expressions of Syn-1,NeuN,PSD-95 were all increased significantly(P＜0.05 or P＜0.01).CONCLUSIONS Gualou guizhi granule can promote synaptic plasticity by activating BDNF/TrkB signaling pathway,thus playing a protective role in cerebral ischemia-reperfusion injury in rats.