生理病理状态下大鼠体内荭草花抗心肌缺血再灌注损伤活性成分筛选OA北大核心CSTPCD

OBJECTIVE To screen the potential active components of Polygonum orientale flower against myocardial ischemia-reperfusion injury(MIRI)in rats based on physiological and pathological states.METHODS SD rats were divided into normal control group,normal administration group,MIRI control group and MIRI administration group,with 5 rats in each group.After drug intervention or modeling and drug intervention,chromatographic separation plasma samples were collected,and chromatographic separation and mass spectrometry data collection were performed by using UPLC-Q-TOF/MS.The prototype components and metabolites were analyzed by comparing the reference substance maps,the maps of each plasma sample,and the relevant literature.At the same time,the common peaks in plasma samples of rats in normal administration group and MIRI administration group were identified.Combined with principal component analysis and orthogonal partial least square-discriminant analysis,the differential transitional components were screened out according to the value of variable importance in the projection(VIP)＞1,to speculate the potential active components of P.orientale flower in rats under physiological and pathological states.The SD rats were divided into control group,MIRI group,positive control group(Compound danshen tablets 0.2 g/kg,3 times a day),and potentially active compound groups(10 mg/kg,twice a day),with 5 rats in each group.The rats in administration groups were given relevant medicine intragastrically,for 3 consecutive days.The activity of superoxide dismutase(SOD),the leakages of lactate dehydrogenase(LDH),creatine kinase isoenzyme-MB(CK-MB)and cardiac troponin Ⅰ(cTnⅠ)in plasma were detected after the last administration.RESULTS Twenty-six main chromatographic peaks were obtained from the total ion chromatogram of the extract of P.orientale flower,and 14 of them were determined,including gallic acid,catechin,protocatechuic acid and so on.There were fifteen(including 6 absorbed prototype components and 9 metabolites)and nineteen transitional components(including 6 absorbed prototype components and 13 metabolites)in the plasma sample of normal rats and MIRI rats.Eight transitional components were detected in both normal rats and MIRI rats,and the VIP values of kaempferol glucuronidation metabolites,quercetin carbonylation metabolites and N-p-paprazine to the corresponding peak were higher than 1.Compared with MIRI group,the activities of SOD were increased significantly in the plasma of MIRI rats in each potential active compound group(P＜0.01),and the leakages of LDH,CK-MB,and cTnⅠ in the plasma of MIRI rats were reduced significantly(P＜0.01).CONCLUSIONS The potential anti-MIRI active components in extract of P.orientale flower are N-p-paprazine,quercetin,kaempferol and kaempferol-3-O-β-D-glucoside.