中国药房2024，Vol.35Issue(16)：2023-2026,4.DOI:10.6039/j.issn.1001-0408.2024.16.15
重症感染患者亚胺培南检测方法的建立及临床应用
Establishment and clinical application of imipenem measurement method in patients with severe infection
摘要
Abstract
OBJECTIVE To establish two-dimensional liquid chromatography method for the determination of imipenem blood concentration and apply it in clinical practice.METHODS The method for the determination of imipenem blood concentration was established based on automatic two-dimensional liquid chromatography.The targets were extracted by 1-dimensional column Aston SNCB(50 mm×4.6 mm,5 μm)and further separated and determined by 2-dimensional column Aston SCB(250 mm×4.6 mm,5 μm).The 1-dimensional mobile phase was imipenem-1D mobile phase[acetonitrile-methanol-water(15∶10∶75,V/V/V)]with a flow rate of 1.0 mL/min;2-dimensional mobile phase was 72%OPI-1 organic mobile phase(chromatographic grade methanol)-20%BPI-1 alkaline mobile phase[water(containing 20.0 mmol/L ammonium phosphate,pH adjusted to 7.2 with triethylamine)]-8%API-1 acidic mobile phase[water(containing 20.0 mmol/L ammonium phosphate,pH adjusted to 3.0 with phosphoric acid)]with a flow rate of 1.0 mL/min;the column temperature was 40℃,UV detection wavelength was 310 nm and injection volume was 100 μL.Elution procedure:1-dimensional column consisted of imipenem-1D mobile phase with eluting for 0-3.40 min;2-dimensional column consisted of 72%OPI-1 organic mobile phase-20%BPI-1 alkaline mobile phase-8%API-1 acidic mobile phase with eluting for 3.40-11.00 min.RESULTS The linear range of imipenem was 0.171-18.570 μg/mL(R 2=0.999 9)with the lower limit of quantification for 0.171 μg/mL;the recovery rate ranged from 93.47%to 106.16%(n=5)and the RSDs of both intra-day and inter-day precision were below 15%(n=5).The minimum concentration of imipenem in 51 patients ranged from 0 to 19.57 μg/mL.CONCLUSIONS The established method is simple and fast with the large scale of sample,and can be used for the imipenem blood concentration monitoring in patients with severe infection.关键词
亚胺培南/血药浓度监测/重症感染/二维液相色谱Key words
imipenem/blood concentration monitoring/severe infection/2D-LC/UV分类
医药卫生引用本文复制引用
陈永妍,孙迪迪,韩文超,王前,张寒娟..重症感染患者亚胺培南检测方法的建立及临床应用[J].中国药房,2024,35(16):2023-2026,4.基金项目
河南省医学科技攻关计划项目(No.LHGJ20220851) （No.LHGJ20220851）
