Abstract
Objective To establish an inductively coupled plasma-mass spectrometry(ICP-MS)method for simultaneous determination of 12 elements in Plantaginis Herba,and to perform chemometric analysis.Methods The radio frequency power was 1800 W,the plasma gas flow rate was 15 L/min,the carrier gas flow rate was 1 L/min,the sampling depth was 12 mm,the auxiliary flow rate was 1 L/min,the atomization chamber temperature was 2.0℃,the measurement mode was kinetic energy discrimination(KED)mode,the collision gas flow rate was 6.0 mL/min,the injection flushing time was 35 s,the peristaltic pump speed was 50 r/min,and the scanning frequency was three times.Based on the contents of 12 elements in 15 batches of Plantaginis Herba from three producing areas,the characteristic spectra were established,and the principal component analysis was performed.Results The linear ranges of cadmium(Cd),lead(Pb),copper(Cu),sodium(Na),zinc(Zn),potassium(K),aluminum(Al),mercury(Hg),calcium(Ca),magnesium(Mg),arsenic(As)and iron(Fe)were in the range of 0-100 ng/mL,0-100 ng/mL,0-500ng/mL,0-500ng/mL,0-1000ng/mL,0-500ng/mL,0-500ng/mL,0-20ng/mL,0-500ng/mL,0-1000ng/mL,0-100ng/mL,0-1000ng/mL(r≥0.9992,n=6)respectively.The RSDs of precision,repeatability and stability tests were all lower than 5%.The average recovery rates of the above 12 elements were in the range of 82.72%-91.10%,with RSDs of 1.55%-4.69%(n=6).The average contents of 12 inorganic elements in 15 batches of Plantaginis Herba were 0.36,1.58,9.16,10.51,54.71,9.35,34.86,0.16,16.81,112.64,1.08,100.04 mg/kg,respectively.The peak shapes of characteristic spectra of 12 elements in Plantaginis Herba from three different producing areas were similar.Mg,Fe,Zn and Al were the characteristic elements of Plantaginis Herba.The quality of 15 batches of Plantaginis Herba was different,and that in Bijie,Guizhou was the best.Conclusion The established ICP-MS method is simple and accurate,which can be used for the content determination of 12 elements in Plantaginis Herba.The characteristic spectra and principal component analysis can provide a reference for the development,utilization and quality control of Plantaginis Herba.关键词
车前草/电感耦合等离子体质谱法/无机元素/特征图谱/主成分分析/含量测定Key words
Plantaginis Herba/inductively coupled plasma-mass spectrometry/inorganic element/characteristic spectra/principal component analysis/content determination分类
医药卫生
