电感耦合等离子体质谱法测定鲜河蚌肉水提物中5种元素含量OACSTPCD

Objective To establish an inductively coupled plasma-mass spectrometry(ICP-MS)method for determining the contents of copper(Cu),arsenic(As),cadmium(Cd),mercury(Hg),and lead(Pb)in the water extract of fresh mussel meat.Methods The samples were pretreated by microwave digestion,and the contents of five elements were determined by the ICP-MS method.The plasma radio frequency power was 1550 W,the plasma gas flow rate was 15.0 L/min,the auxiliary gas(argon)flow rate was 0.90 L/min,the atomization gas flow rate was 1.05 L/min,the peristaltic pump speed was 0.1 r/s,the sampling depth was 8 mm,the tuning mode was No Gas mode,the repetition frequency was three times.Results The linear ranges of Cu,As,Cd,Hg,and Pb were in the range of 0-500 ng/mL,0-200 ng/mL,0-10 ng/mL,0-5 ng/mL,and 0-20 ng/mL(r≥0.9996,n=5)respectively.The limits of detection(LOD)were 0.0205,0.0045,0.0008,0.0042,0.0051 mg/kg respectively,the limits of quantification(LOQ)were 0.0682,0.0149,0.0027,0.0141,0.0170 mg/kg respectively.The RSDs of precision and repeatability tests were lower than 5%.The average recovery rates of the above five elements were 99.60%,106.16%,108.93%,115.30%,103.40%with RSDs of 0.84%,6.03%,1.15%,3.56%,0.55%(n=6)respectively.Based on the Green Standards for Medicinal Plants and Preparations for Foreign Trade and Economy,Cu,Hg and Pb in nine batches of samples met the limits,while As and Cd levels were over the limits in eight and two batches of samples respectively.Conclusion This method is simple and accurate,which can be used to determine the contents of Cu,As,Cd,Hg,and Pb in the water extract of fresh mussel meat.