中华临床免疫和变态反应杂志2024，Vol.18Issue(4)：376-382,7.DOI:10.3969/j.issn.1673-8705.2024.04.008
蒿属花粉过敏性哮喘小鼠模型的建立
Establishment of mouse model of Artemisia pollen-allergic asthma
摘要
Abstract
Objective To establish mouse model of Artemisia pollen-allergic asthma.Methods Twelve pathogen-free female BALB/c mice were randomly divided into control and model groups.Mice in model group were sensitized once per week for 4 weeks,then nasal drops were administered daily since 25th to 29th days after last sensitization.Mice were killed and blood samples were collected for serum total immunoglobulin E(T-IgE)and Artemisia pollen specific immunoglobulin E(sIgE)detection after evaluations of airway resistance and lung compliance on 30th day.Total number of leukocytes,differential leukocyte count and cytokines levels of bronchoalveolar lavage fluid(BALF)were also detected.Finally,pathological analysis of lung tissue was performed.Allergens of Artemisia pollen were isolated and identified by using sodium dodecyl sulfate poly acryl-amide gel electrophoresis(SDS-PAGE)and Western blot analysis(WB).Results Elevated airway resist-ance and decreased lung compliance demonstrated among mice in model group,while serum levels of T-IgE and sIgE as well as eosinophils,neutrophils,IL-4,IL-5,IL-9 and IL-13 in BALF significantly increased,com-pared with that of mice in control group.Bronchial stenosis,infiltration of inflammatory cells,destruction of lung structure and hyperplasia of cup cells were revealed in lung tissues of mice in model group by using Hema-toxylin-eosin(HE)and periodicacid-schiff reaction(PAS)staining.Results of Western blotting showed that sIgE antibodies of mice in model group mainly bound to 9 kDa to 70 kDa proteins bands,whereas serum sIgE antibodies of Artemisia pollen-allergic patients were mainly bound to 28 kDa and 70 kDa proteins bands.Conclusion A mouse model of Artemisia pollen-allergic asthma was successfully established,which can be used for research of pathogenesis and allergen specific immunotherapy of Artemisia pollen-allergic asthma.关键词
过敏性哮喘/动物模型/蒿属花粉Key words
allergic asthma/animal model/artemisia pollen引用本文复制引用
郑卉爽,李俊达,茹一,尚腾泽,关凯..蒿属花粉过敏性哮喘小鼠模型的建立[J].中华临床免疫和变态反应杂志,2024,18(4):376-382,7.基金项目
国家自然科学基金(82070033,82370041) （82070033,82370041）
中国医学科学院医学与健康科技创新工程(2021-I2M-1-017) National Natural Science Foundation(82070033,82370041) （2021-I2M-1-017）
Medical and Health Science and Technology Innovation Project of Chinese Academy of Medical Sciences(2021-I2M-1-017) （2021-I2M-1-017）
