中华临床免疫和变态反应杂志2024，Vol.18Issue(4)：383-388,6.DOI:10.3969/j.issn.1673-8705.2024.04.009
乳酸酚棉兰染色在气传花粉鉴定中的应用
Application of lactophenol cotton blue staining in identification of airborne pollens
摘要
Abstract
Objective To explore the potential application of lactophenol cotton blue(LPCB)staining method in identification of airborne pollen under microscope.Methods The major airborne pollens of spring and autumn in Weifang City was identified by using basic fuchsin(BF)staining and LPCB staining and results of daily pollen count were recorded.Results The results of this study demonstrated that LPCB staining showed obvious advantages in identification of Broussonetia papyrifera,Platanus,Artemisia,Amaranthacae and Gra-mineae pollens,compared with BF staining.However,it showed disadvantage in identification of CUPRESSACEAE pollen.In addition,the major airborne pollens of summer and autumn have not significant difference within last 40 years in Weifang.Due to alternation of planting tree species,Platanus pollen has be-come the major allergenic pollen in spring.Conclusion LPCB staining can be used for identification of fungi and airborne pollens concurrently,which may ensure accuracy of identification as well as saving time costs.关键词
气传花粉/镜检/碱性复红/乳酸酚棉兰/染色Key words
airborne pollen/microscopy/lactophenol cotton blue/basic fuchsin/staining引用本文复制引用
张续德,冯少杰,刘菁菁,王昱琳,刘凤霞,张芹..乳酸酚棉兰染色在气传花粉鉴定中的应用[J].中华临床免疫和变态反应杂志,2024,18(4):383-388,6.基金项目
山东省医药卫生科技项目(202303111109) （202303111109）
潍坊市卫健委计划项目(WFWSJK-2021-004,WFWSJK-2021-225) （WFWSJK-2021-004,WFWSJK-2021-225）
潍坊市科技发展计划项目(2023YX002) Shandong Medical and Health Science&Technology Foundation(202303111109) （2023YX002）
Weifang Health Commission Funding(WFWSJK-2021-004,WFWSJK-2021-225) （WFWSJK-2021-004,WFWSJK-2021-225）
Weifang Science&Tech-nology Development Project(2023YX002) （2023YX002）
