肿瘤防治研究2024，Vol.51Issue(8)：667-677,11.DOI:10.3971/j.issn.1000-8578.2024.23.1372
miR-18a-5p靶向调控RORA在结直肠癌增殖进展中的作用及临床意义
miR-18a-5p Regulates Colorectal Cancer Proliferation and Progression by Targeting RORA
摘要
Abstract
Objective To investigate the mechanism and clinical significance of miR-18a-5p and retinoid acid receptor-related orphan receptor-α(RORA)in the proliferation and progression of colorectal cancer(CRC)cells.Methods The expressions of miR-18a-5p and RORA in CRC cells and tissues were detected via qRT-PCR,FISH,and IHC.Cell proliferation capability was detected through EdU and CFSE assay,cell apoptosis by flow cytometry assay,and cell migration and invasion abilities by cell scratch and Transwell invasion assays,respectively.The targeted regulation of miR-18a-5p on RORA was further verified via dual-luciferase reporter assay,cell function rescue test,RT-PCR,and Western blot assay.Finally,bioinformatics was used to explore the molecular mechanism of miR-18a-5p promoting malignant proliferation,invasion,and progression of CRC via regulating RORA.Results miR-18a-5p exhibited a high expression in CRC tissues and cells(P<0.05)and promoted the proliferation,migration,and invasion of CRC cells(P<0.05).In addition,RORA served as the target gene of miR-18a-5p,and its overexpression effectively reduced the promoting function of miR-18a-5p in the malignant biological phenotype of CRC cells(P<0.05).The expression of RORA in CRC tissues showed a significantly positively correlation with the infiltration of CD8+T cells and the expression of its surface marker protein CD8A.Conclusion The targeted regulation of RORA by miR-18a-5p promotes the proliferation and progression of CRC.The miR-18a-5p/RORA regulatory pathway possibly contributes to the immune microenvironment of CRC,which can be a potential therapeutic target for CRC.关键词
miR-18a-5p/RORA/结直肠癌/增殖Key words
miR-18a-5p/RORA/Colorectal cancer/Proliferation分类
医药卫生引用本文复制引用
陈一锋,王涛,王帅,柴明明,张迪,王春霞,赵丽霞,张洪来,杨熊飞,张维胜..miR-18a-5p靶向调控RORA在结直肠癌增殖进展中的作用及临床意义[J].肿瘤防治研究,2024,51(8):667-677,11.基金项目
National Natural Science Foundation of China(No.82160522) （No.82160522）
National Health Commission Gastrointestinal Tumor Diagnosis and Treatment Key Laboratory Project(No.NLDTG2020021) （No.NLDTG2020021）
Chinese Academy of Medical Sciences Central Level Public Welfare Research Institutes Basic Research Funds Special Project(No.2019PT320005) （No.2019PT320005）
Natural Science Foundation of Gansu Province(No.22JR5RA653) （No.22JR5RA653）
Lanzhou Science and Technology Project(No.2022-5-68)国家自然科学基金(82160522) （No.2022-5-68）
国家卫生健康委员会胃肠道肿瘤诊疗重点实验室项目(NLDTG2020021) （NLDTG2020021）
中国医学科学院中央级公益性科研院所基本科研业务费专项项目(2019PT320005) （2019PT320005）
甘肃省自然科学基金(22JR5RA653) （22JR5RA653）
兰州市科技计划项目(2022-5-68) （2022-5-68）
