中国现代医生2024，Vol.62Issue(22)：58-62,5.DOI:10.3969/j.issn.1673-9701.2024.22.014
miR-204和SIRT1在结直肠癌中的表达情况及临床意义
Expression of miR-204 and SIRT1 in colorectal cancer and their clinical significance
黄立勇 1吴佳明 1彭玉平 1李进 1陈治横1
作者信息
- 1. 嘉兴大学附属医院胃肠外科,浙江嘉兴 314000
- 折叠
摘要
Abstract
Objective To investigate the expression of miR-204 and silence information regulator 1(SIRT1)in colorectal cancer and their clinical value.Methods Cancer tissue specimens and paracancer tissue specimens of 60 patients with colorectal cancer treated in Department of Gastrointestinal Surgery of Affiliated Hospital of Jiaxing University from May 2018 to June 2020 were collected as study objects.Real time fluorogenic quantitative polymerase chain reaction was used to detect the gene expression of miR-204 and SIRT1,and the correlation between miR-204 and SIRT1 gene expression was compared by Pearson analysis.Immunohistochemical SABC method was used to detect SIRT1 protein expression,and relationship between different SIRT1 protein expression and clinicopathological features was compared.Kaplan-Meier method was used to analyze the survival difference of colorectal cancer patients with different SIRT1 protein expression.Results The mRNA expression of miR-204 gene in cancer tissues was significantly lower than that in paracancer tissues(P<0.05),and the mRNA expression of SIRT1 gene was significantly higher than that in paracancer tissues(P<0.05).Pearson correlation analysis showed that miR-204 was negatively correlated with SIRT1 mRNA expression in both paracancer tissues and cancer tissues(r=-0.647,-0.737,P<0.05).The expression of SIRT1 protein was correlated with the differentiation level,invasion level,lymph node metastasis and TNM stage of colorectal cancer(P<0.05),but not with patient age,gender,tumor size and tumor site(P>0.05).Kaplan-Meier analysis showed that the survival rate of patients with positive expression of SIRT1 in cancer tissues was significantly lower than that of patients with negative expression of SIRT1(χ2=5.001,P=0.025).Conclusion The expression of miR-204 is down-regulated and SIRT1 expression is up-regulated in colorectal cancer tissues,which may jointly promote the metastasis and invasion of colorectal cancer and affect the prognosis of patients through mutual influence.关键词
结直肠癌/miR-204/沉默信息调节因子1/基因表达Key words
Colorectal cancer/miR-204/Silence information regulator 1/Gene expression分类
医药卫生引用本文复制引用
黄立勇,吴佳明,彭玉平,李进,陈治横..miR-204和SIRT1在结直肠癌中的表达情况及临床意义[J].中国现代医生,2024,62(22):58-62,5.
