根癌农杆菌介导的蒙古冰草稳定遗传转化体系建立OA北大核心CSTPCD

Agropyron mongolicum is a perennial shrub grass in the Triticeae tribe of Poaceae family.It has high forage quality and tolerance to cold,drought,salt and sandstorm.Therefore,it is an excellent grass species for mining stress tolerant genes and restoring deteriorated grasslands.However,a highly efficient transformation system has yet to be established in A.mongolicum.It restricts the identification of genetic resources and the application of genetic improve-ment of this species.Here,we report an embryogenic callus line(ECL)#89 that was induced from the seeds of A.mon-golicum cultivar Mengnong No.1.Our studies showed the ECL #89 had high regeneration and Agrobacterium-infection efficiencies.An Agrobacterium-mediated transformation system with a transformation efficiency up to 30％was estab-lished by infecting the ECL #89 with EHA105.Additionally,we found that subcultures could decrease severely the re-generation capacity of ECL #89,which could be partially restored by adding(1 mg·L-1)ABA or high concentration(45 g·L-1)sucrose in the regeneration medium,from 5％to 35％and 42％,respectively.Our work will facilitate the de-veloping of genome editing system,gene function characterization,and molecular breeding in A.mongolicum.