植物学报2024，Vol.59Issue(4)：600-612,13.DOI:10.11983/CBB24012
根癌农杆菌介导的蒙古冰草稳定遗传转化体系建立
Establishment of Agrobacterium-mediated Transformation System for Agropyron mongolicum
摘要
Abstract
Agropyron mongolicum is a perennial shrub grass in the Triticeae tribe of Poaceae family.It has high forage quality and tolerance to cold,drought,salt and sandstorm.Therefore,it is an excellent grass species for mining stress tolerant genes and restoring deteriorated grasslands.However,a highly efficient transformation system has yet to be established in A.mongolicum.It restricts the identification of genetic resources and the application of genetic improve-ment of this species.Here,we report an embryogenic callus line(ECL)#89 that was induced from the seeds of A.mon-golicum cultivar Mengnong No.1.Our studies showed the ECL #89 had high regeneration and Agrobacterium-infection efficiencies.An Agrobacterium-mediated transformation system with a transformation efficiency up to 30％was estab-lished by infecting the ECL #89 with EHA105.Additionally,we found that subcultures could decrease severely the re-generation capacity of ECL #89,which could be partially restored by adding(1 mg·L-1)ABA or high concentration(45 g·L-1)sucrose in the regeneration medium,from 5％to 35％and 42％,respectively.Our work will facilitate the de-veloping of genome editing system,gene function characterization,and molecular breeding in A.mongolicum.关键词
蒙古冰草/胚性愈伤组织/根癌农杆菌/遗传转化Key words
Agropyron mongolicum/embryogenic callus/Agrobacterium tumefaciens/genetic transformation引用本文复制引用
李宇琛,赵海霞,姜希萍,黄馨田,刘亚玲,吴振映,赵彦,付春祥..根癌农杆菌介导的蒙古冰草稳定遗传转化体系建立[J].植物学报,2024,59(4):600-612,13.基金项目
内蒙古自治区种业科技创新重大示范工程"揭榜挂帅"项目(No.2022JBGS0014)、中央引导地方科技发展资金(No.2022ZY006)、青岛新能源山东实验室"抓攻关"项目(No.QNESLKPP202302)、国家林业和草原局重大应急科技"揭榜挂帅"项目(No.202201)和呼和浩特市重大科技专项(No.2021-重-社-2) （No.2022JBGS0014）
