植物学报2024，Vol.59Issue(4)：626-634,9.DOI:10.11983/CBB23118
捕虫堇叶片高效再生体系建立
Establishment of an Efficient Leaf Regeneration System for Pinguicula
摘要
Abstract
Using two different genotypes of Pinguicula cyclosecta and P.'Sethos'as explants,an efficient system for adventitious bud regeneration of Pinguicula was established by studying the factors affecting regeneration.The results showed that the two kinds of Pinguicula had the same requirements for the disinfection scheme and basic medium,but the regeneration ability differed.The best medium for P.cyclosecta leaf regeneration was MS+1.0 mg·L-1 6-BA+0.2 mg·L-1 NAA,with a regeneration rate of 92.22％and a regeneration coefficient was 4.84.On MS+0.6 mg·L-1 6-BA+0.1 mg·L-1 NAA media,the proliferation coefficient of adventitious buds reached 4.98.The best medium for the leaf regeneration of P.'Sethos'was MS+2.0 mg·L-1 6-BA+0.2 mg·L-1 NAA,with a regeneration rate of 77.78％and a regeneration coefficient of 6.12.On the MS+0.3 mg·L-1 6-BA+0.1 mg·L-1 NAA media,the proliferation coefficient reached 4.84.After root culture(1/2MS+0.1 mg·L-1 IBA),the root state of the two kinds of Pinguicula was the best.This study solved the problems of a low reproduction coefficient and difficulty in factory breeding of Pinguicula and provided technical support for its large-scale production and breeding improvement.关键词
食虫植物/捕虫堇属/叶片再生/不定芽Key words
carnivorous plant/Pinguicula/leaf regeneration/adventitious bud引用本文复制引用
杨佳丽,饶羽菲,张润花,周国林,林处发,何燕红,宁国贵..捕虫堇叶片高效再生体系建立[J].植物学报,2024,59(4):626-634,9.基金项目
现代农业产业技术体系(No.CARS-23) （No.CARS-23）
