安徽农业科学2024，Vol.52Issue(16)：1-5,5.DOI:10.3969/j.issn.0517-6611.2024.16.001
海水中降解几丁质细菌的筛选鉴定及培养条件优化
Screening and Identification of Chitin Degrading Bacteria in Seawater and Optimizing Culture Conditions
摘要
Abstract
[Objective]To obtain high-yield chitinase strains from seawater and optimize culture conditions.[Method]The seawater from Jin-sha Bay of Zhanjiang City was collected as samples,chitinase producing strains were isolated using colloidal chitin as the only carbon source,the enzyme activity was measured by DNS method and molecular identification;single factor experiment and orthogonal optimization were used to optimize the optimal conditions for enzyme production.[Result]Six strains of chitinase-degrading bacteria were screened.The strain with the strongest chitinase activity was Z-4.The strain was identified as Stenotrophomonas maltophilia by morphological observation and 16S rDNA se-quence analysis.The optimum conditions for producing enzyme of strain Z-4 were obtained as follows:soluble starch addition of 1.5%,peptone addition of 2.5%,KH2 PO4 addition of 0.04%;at 30℃,pH 7.0,inoculation amount of 2%,160 r/min and 72 hours of cultivation,the enzyme activity reached 0.452 U/mL,which was 5 times higher than before optimization.[Conclusion]The research results provide an important refer-ence for the effective use of chitin in the ocean to improve the environment.关键词
海水/细菌/几丁质酶/培养条件优化/筛选鉴定Key words
Seawater/Bacteria/Chitinase/Optimization of culture conditions/Screening and identification分类
农业科技引用本文复制引用
祁红兵,张敏怡..海水中降解几丁质细菌的筛选鉴定及培养条件优化[J].安徽农业科学,2024,52(16):1-5,5.基金项目
湛江市非资助科技攻关计划项目(2023B01023) （2023B01023）
岭南师范学院校级教育教学改革项目(岭师教务[2022]154号). （岭师教务[2022]154号）
