安徽农业科学2024，Vol.52Issue(16)：91-95,100,6.DOI:10.3969/j.issn.0517-6611.2024.16.019
加州鲈p65基因克隆及表达分析
Cloning and Expression Analysis of p65 Gene in Micropterus salmoides
摘要
Abstract
Cloning and obtaining the coding region sequence of the p65 gene in the NF-κ B family from the brain tissue of Micropterus salmo-ides,with a total cDNA length of 1 944 bp,the ORF is 1 899 bp and can encode 632 amino acids.The theoretical molecular weight is 69.61 ku,and the theoretical isoelectric point(PI)is 5.66.The protein structure of the p65 gene was analyzed using bioinformatics analysis tools,and its protein function was predicted.The results showed that the gene does not contain a signal peptide sequence and does not have a transmembrane domain,but there is a conserved domain in the NF-κ B family.The results of multiple sequence alignment show that the simi-larity between Micropterus salmoides p65 and Micropterus dolomieu is the highest,at 99.30%;constructing a phylogenetic tree,it was discov-ered that the Micropterus salmoides p65 and Micropterus dolomieu clustered together.The results of qRT PCR indicate that the Micropterus salmo-ides p65 gene is expressed in all selected tissues,with the highest expression level in brain tissue,followed by the head kidney,thymus,and heart,and the lowest expression level in spleen tissue.关键词
加州鲈/p65/生物信息学/组织表达分析Key words
Micropterus salmoides/p65/Bioinformatics/Organizational expression analysis分类
水产学引用本文复制引用
丘金珠,唐怀庆,宋海霞,赵天珍,梁富,邓晓华,梁莹,宋长江..加州鲈p65基因克隆及表达分析[J].安徽农业科学,2024,52(16):91-95,100,6.基金项目
中山市2021年第二批社会公益与基础研究项目"加州鲈虹彩病毒病发病规律与防控技术研究"(2021B2056). （2021B2056）
