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桦褐孔菌口服佐剂对禽类疫苗的体液免疫增强作用研究

杨硕黄悦妍刘娜刘建钗费智国郭梦帅郭玲陈智张琳丁志勇关红艳赵晓明

安徽农业科学2024，Vol.52Issue(17)：94-97,4.

安徽农业科学2024，Vol.52Issue(17)：94-97,4.DOI:10.3969/j.issn.0517-6611.2024.17.020

桦褐孔菌口服佐剂对禽类疫苗的体液免疫增强作用研究

Study on the Enhancement Effect of Oral Adjuvant of Inonotus obliquus on the Humoral Immunity of Avian Vaccines

杨硕 ¹黄悦妍 ²刘娜 ¹刘建钗 ¹费智国 ²郭梦帅 ³郭玲 ⁴陈智 ⁵张琳 ⁵丁志勇 ⁶关红艳 ⁶赵晓明⁷

作者信息

1. 河北工程大学生命科学与食品工程学院,河北邯郸 056038
2. 河北工程大学生命科学与食品工程学院,河北邯郸 056038||山东省农业科学院畜牧兽医研究所/山东省畜禽疫病防治与繁育重点实验室,山东济南 250100||农业农村部畜禽生物组学重点实验室,山东济南 250100
3. 山东省农业科学院畜牧兽医研究所/山东省畜禽疫病防治与繁育重点实验室,山东济南 250100||农业农村部畜禽生物组学重点实验室,山东济南 250100||山东农业大学动物科技学院(动物医学院),山东泰安 271018
4. 济南市公园发展服务中心济南动物园工作部,山东济南 250100
5. 山东省农业科学院畜牧兽医研究所/山东省畜禽疫病防治与繁育重点实验室,山东济南 250100||农业农村部畜禽生物组学重点实验室,山东济南 250100
6. 秦皇岛高通生物科技有限公司,河北昌黎 066600
7. 昌黎县动物防疫监督站城关分站,河北昌黎 066600
折叠

摘要

Abstract

In order to clarify the applicability of Inonotus obliquus fermentation product(IOFP)as the adjuvants of avain vaccines,commer-cial avian influenza inactivated vaccine and duck Tembusu virus attenuated live vaccine were selected to immunize SPF chicken and ducks,re-spectively.SPF chicken in the experimental group were fed with the commercial diet containing 0.8%IOFP,SPF chicken in the control group were fed with the the commercial diet.The peripheral blood from the chicken and ducks was collected at different time points after immuniza-tion and the serum was separated to determine the specific hemagglutinin inhibition(HI)antibodies of avian influenza virus(AIV),specific antibodies of duck Tembusu virus(DTMUV),and their neutralizing antibody titer.The effects of IOFP on the humoral immune response were evaluated by using the statistical methods.After 28 days post immunization,the ducks were challenged with virulent DTMUV,and the cloacal detoxification volume was detected,so as to confirm the effects of IOFP on the detoxification volume of DTMUV.The results showed that IOFP as oral adjuvant could significantly improve the titers of specific and neutralizing antibodies against AIV and DTMUV,and especially the im-provement effect on the antibodies at the early stage.In addition,adding IOFP could reduce the detoxification volume of DTMUV,and shorten the shedding time.Therefore,IOFP was a common oral adjuvant for avian viral vaccines,which had a great improvement effect on shortening the immune"gap period"and improving the protective effect of vaccines.

关键词

桦褐孔菌发酵产物/禽流感病毒灭活疫苗/鸭坦布苏病毒弱毒活疫苗/抗体/中和抗体

Key words

Inonotus obliquus fermentation product/Avian influenza inactivated vaccine/Duck Tembusu virus attenuated vaccine/Antibody/Neutralizing antibody

分类

农业科技

引用本文复制引用

杨硕,黄悦妍,刘娜,刘建钗,费智国,郭梦帅,郭玲,陈智,张琳,丁志勇,关红艳,赵晓明..桦褐孔菌口服佐剂对禽类疫苗的体液免疫增强作用研究[J].安徽农业科学,2024,52(17):94-97,4.

基金项目

山东省重点研发计划项目"乡村振兴科技创新提振行动计划"(2022TZXD0041) （2022TZXD0041）

秦皇岛市珍稀药用真菌应用创新平台项目(202201B051) （202201B051）

山东省重点研发计划项目(竞争性创新平台)(2022CXPT005-02,2022CXPT010) （竞争性创新平台）

山东省农业科学院农业科技创新工程项目(CXGC2023A21). （CXGC2023A21）

安徽农业科学

ISSN：0517-6611

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