基于新疆乳用型褐牛遗传缺陷基因和致死单倍型检测应用效果研究OA

In order to improve the breeding level of Xinjiang dairy brown cattle,this study used primer combinations and reagent kits of 93 ge-netic defect genes and lethal haplotypes in cattle,and randomly sampled 296 dairy type Xinjiang brown cattle to detect causal mutation sites(SNPs,short segment insertions or deletions)and lethal haplotypes(BH2,BH6,BH14,OH2,OH4,etc.)of genetic defect genes(such as spinal muscular atrophy,myelin dysplasia,spider leg syndrome,etc.)and lethal haplotypes(BH2,BH6,BH14,OH2,OH4,etc.).Carri-ers of single gene genetic defect genes and carriers of lethal haplotypes were screened out,indicating that BH2,BH6,BH14,and abnormal spinal cord myelin development have a certain proportion in the population of Xinjiang brown cattle mother cows.The statistical analysis of re-productive performance and disease incidence between genetic defect genes and lethal haplotypes in cattle showed significant differences(P<0.05);breeding and mating methods were used to breed and produce normal F1 generation(no defective genes or lethal haplotypes were de-tected)for carrying cattle.The reproductive indicators were analyzed,and the results showed a significant improvement in reproductive per-formance.Therefore,it is necessary to screen the carrier cows regularly and select and match them reasonably to provide basis for breeding of milk performance,population genetic improvement and protection of genetic resources of Xinjiang brown cattle.