安徽农业科学2024，Vol.52Issue(17)：98-100,104,4.DOI:10.3969/j.issn.0517-6611.2024.17.021
基于新疆乳用型褐牛遗传缺陷基因和致死单倍型检测应用效果研究
Research on the Application Effect of Genetic Defect Denes and Lethal Haplotype Detection in Xinjiang Dairy Brown Cattle
摘要
Abstract
In order to improve the breeding level of Xinjiang dairy brown cattle,this study used primer combinations and reagent kits of 93 ge-netic defect genes and lethal haplotypes in cattle,and randomly sampled 296 dairy type Xinjiang brown cattle to detect causal mutation sites(SNPs,short segment insertions or deletions)and lethal haplotypes(BH2,BH6,BH14,OH2,OH4,etc.)of genetic defect genes(such as spinal muscular atrophy,myelin dysplasia,spider leg syndrome,etc.)and lethal haplotypes(BH2,BH6,BH14,OH2,OH4,etc.).Carri-ers of single gene genetic defect genes and carriers of lethal haplotypes were screened out,indicating that BH2,BH6,BH14,and abnormal spinal cord myelin development have a certain proportion in the population of Xinjiang brown cattle mother cows.The statistical analysis of re-productive performance and disease incidence between genetic defect genes and lethal haplotypes in cattle showed significant differences(P<0.05);breeding and mating methods were used to breed and produce normal F1 generation(no defective genes or lethal haplotypes were de-tected)for carrying cattle.The reproductive indicators were analyzed,and the results showed a significant improvement in reproductive per-formance.Therefore,it is necessary to screen the carrier cows regularly and select and match them reasonably to provide basis for breeding of milk performance,population genetic improvement and protection of genetic resources of Xinjiang brown cattle.关键词
新疆褐牛/遗传缺陷/基因Key words
Xinjiang brown cattle/Genetic defects/Gene分类
农业科技引用本文复制引用
蒋晓新,高翔,刘棚菲,鞠志花,刘江卫,许芸..基于新疆乳用型褐牛遗传缺陷基因和致死单倍型检测应用效果研究[J].安徽农业科学,2024,52(17):98-100,104,4.基金项目
2022年度新疆农业职业技术学院资助项目(XJNZYKJ-202211) （XJNZYKJ-202211）
2023年昌吉州科技特派员农村科技创业行动项目(服务产业类6). （服务产业类6）
