北京中医药大学学报2024，Vol.47Issue(8)：1089-1099,11.DOI:10.3969/j.issn.1006-2157.2024.08.010
基于UPLC-Q-Exactive Orbitrap MS结合网络药理学的雪菊黄酮部位入血成分及其血脂调节机制研究
Study on blood components and blood lipid regulation mechanism of Coreopsis tinctoria Nutt. flavones based on UPLC-Q-Exactive Orbitrap MS combined with network pharmacology
摘要
Abstract
Objective To investigate the potential active ingredients and the mechanism of Coreopsis tinctoria Nutt. in the prevention and treatment of hyperlipidemia. Methods Ultra-high performance liquid chromatography-Quadrupole-Exactive Orbitrap mass spectrometry (UPLC-Q-Exactive Orbitrap MS) was used to qualitatively analyze the fractions and blood components of flavones in Coreopsis tinctoria Nutt. The intersection targets of flavones in Coreopsis tinctoria Nutt. and hyperlipidemia were screened,and the protein-protein interaction network was constructed and analyzed by the STRING 12.0 database. Finally,the gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) were used for enrichment analysis. Results A total of 25 compounds were detected from the flavones in Coreopsis tinctoria Nutt.,and their structures were identified,including ten chalcones,nine flavanones,four flavonols,one aurone,and one biflavone. The analysis of blood components showed that marein,flavanomarein,okanin,isookanin and 5,7,3',5'-tetrahydroxyflavanone-7-O-β-D-glucopyranoside were the main components of the flavones in Coreopsis tinctoria Nutt. in blood. Network pharmacological GO and KEGG enrichment analysis showed that the flavones in Coreopsis tinctoria Nutt. may regulate phosphatidylinositol 3-kinase/protein kinase B,tumor necrosis factor,hypoxia-inducible factor-1 signaling pathway and other signaling pathways in the regulation and prevention of hyperlipidemia. Conclusion Coreopsis tinctoria Nutt. can prevent and treat hyperlipidemia,and the mechanism may be related to the five blood components of the flavones in Coreopsis tinctoria Nutt.,including marein,flavanomarein,okanin,isookanin and 5,7,3',5'-tetrahydroxyflavanone-7-O-β-D-glucopyranoside.关键词
雪菊/高脂血症/超高效液相色谱串联四极杆静电场轨道阱质谱/入血成分/网络药理学Key words
Coreopsis tinctoria Nutt./hyperlipidemia/ultra-high performance liquid chromatography-Quadrupole-Exactive Orbitrap mass spectrometry/blood component/network pharmacology分类
医药卫生引用本文复制引用
曹前,魏胜利,张静怡,陈万金,王越,邵炜娴,张媛..基于UPLC-Q-Exactive Orbitrap MS结合网络药理学的雪菊黄酮部位入血成分及其血脂调节机制研究[J].北京中医药大学学报,2024,47(8):1089-1099,11.基金项目
国家重点研发计划"中医药现代化研究"专项(No.2017YFC1700702) "Traditional Chinese Medicine Modernization Research"Special Program of National Key R&D Program(No.2017YFC1700702) （No.2017YFC1700702）
