滨州医学院学报2024，Vol.47Issue(4)：292-296,5.DOI:10.19739/j.cnki.issn1001-9510.2024.04.012
当归芍药散对大鼠盆腔炎性疾病后遗症模型的药效机制
Pharmacological mechanism of Danggui Shaoyao Powder in treating the SPID in rats
摘要
Abstract
Objective To investigate the effect mechanism of Danggui Shaoyao Powder on SPID model of rat pelvic inflam-matory disease.Methods Seventy female SPF SD rats were selected as the experiment subjects,and SPID model of rats was es-tablished by mechanical injury+mixed bacterial solution inoculation.The rats were randomly divided into the blank control group,the sham operation group,the model group,the Danggui Shaoyao Powder high-dose,medium-dose and low-dose groups and the Fuke Qianjin Capsule group,with 10 rats in each group.The morphological changes of uterus in each group were ob-served under the microscope after 20 days of administration.The activity of superoxide dismutase(SOD)and the content of ma-londialdehyde(MDA)in serum of rats were determined by colorimetry.Serum levels of inflammatory cytokines interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α),interleukin-4(IL-4)and interleukin-10(IL-10)were determined by ELISA.Re-sults The optical microscope observation showed that the high-dose and medium-dose groups of Danggui Shaoyao Powder could reduce the inflammatory cell infiltration of the endometrium of rats and promote the proliferation and repair of the pathological epithelial cells.Compared with that in the blank group,the positive score of the uterine pathology of rats in the model group in-creased,while the score of the uterine pathology of rats in the high-dose and medium-dose groups of Danggui Shaoyao Powder de-creased compared with that in the model group.Compared with those in the blank group,the contents of MDA,IL-1β and TNF-α in serum in the model group increased,while the contents of SOD,IL-4 and IL-10 decreased(P＜0.05).Compared with that in the model group,MDA content in serum of rats in Danggui Shaoyao Powder high dose group,medium dose group and Fuke Qianjin capsule group decreased,SOD activity of rats in administration groups increased(P＜0.05),serum IL-1 β and TNF-αcontents of rats in Danggui Shaoyao Powder high dose group and low dose group decreased.The contents of IL-4 and IL-10 in ser-um of rats in each administration group increased(P＜0.05).Conclusion Danggui Shaoyao Powder can regulate the balance of oxidative stress system and the levels of anti-inflammatory and pro-inflammatory factors in the immune system,effectively in-hibit the progression of inflammation,regulate the immune function of human body,and promote the repair of inflammatory tis-sue.关键词
当归芍药散/盆腔炎性疾病后遗症/氧化应激系统/炎症因子Key words
Danggui Shaoyao Powder/sequela of pelvic inflammatory disease/oxidative stress system/inflammatory factor分类
医药卫生引用本文复制引用
张小雪,孙聪聪,张英姿,周超..当归芍药散对大鼠盆腔炎性疾病后遗症模型的药效机制[J].滨州医学院学报,2024,47(4):292-296,5.基金项目
山东省中医药科技项目面上项目(2020M085) （2020M085）
