草业学报2024，Vol.33Issue(8)：145-158,14.DOI:10.11686/cyxb2023346
燕麦sHSP基因家族的鉴定及其响应高温及老化的表达分析
Identification of the oat sHSP gene family and its transcript profiles in response to high temperature and aging
摘要
Abstract
Small heat shock proteins (sHSPs) are a type of protein that is encoded by nuclear genes in plants.The sHSPs are ubiquitous in plants,contain conserved ACD domains,and play important roles in aging as well as in responses to stresses such as high temperature and drought.In this study,24 HSP20 (AsHSP20.1-AsHSP20.24) genes were identified in the oat genome using bioinformatics methods.The physical and chemical properties,protein structure,subcellular localization,phylogeny,conserved motifs,and conserved domains of the putative AsHSP20 family members were determined,and the chromosomal locations of their encoding genes and gene transcription profiles in response to high temperature and aging were systematically analyzed.The AsHSP20 genes were distributed on 17 chromosomes.The genes encoded polypeptides consisting of 136-529 amino acids,with molecular weights ranging from 14.9 to 58.1 kDa,and theoretical isoelectric points ranging from 5.30 to 8.79.Most HSP20 members were predicted to localize in the nucleus,cytoplasm,or chloroplast,and a few were predicted to localize in the plasma membrane,mitochondria,peroxisome,or extracellular space.Protein secondary and tertiary structure analyses showed that AsHSP20 members have β-folded structures.Based on analyses of conserved motif composition and phylogenetic relationships,the AsHSP20 gene family was classified into 11 subgroups.The conserved motifs were similar or identical among the members of the same subgroup,indicative of functional similarity among proteins in the same subgroup.Further analysis of AsHSP20 transcript profiles in seeds showed that AsHSP20.20 and AsHSP20.24 were down-regulated under both natural and artificial aging conditions.It is speculated that AsHSP20.20 and AsHSP20.24 are involved in regulating the reduction in oat seed viability during aging,and are candidate genes related to oat seed longevity.These genes have potential applications in anti-aging germplasm breeding.The results of this study provide valuable information on the regulatory mechanism of the AsHSP20 gene family in the aging process of oat,and also provide theoretical support for further research on oat HSP20 genes and the molecular mechanism of delayed aging in oat seeds.关键词
燕麦/种子老化/HSP20基因家族/系统进化/表达分析Key words
oat/seed aging/HSP20 gene family/phyletic evolution/expression analysis引用本文复制引用
马圆,刘欢,赵桂琴,王敬龙,张然,姚瑞瑞..燕麦sHSP基因家族的鉴定及其响应高温及老化的表达分析[J].草业学报,2024,33(8):145-158,14.基金项目
草业生态系统教育部重点实验室开放课题(KLGE-2022-16),国家自然科学基金(32360344),草种创新与草地农业生态系统全国重点实验室开放基金课题(SKLHIGA202301),甘肃农业大学青年导师基金(GAU-QDFC-2023-01),西藏自治区科技计划项目(XZ202201ZY0014N)和国家自然青年基金(32301491)资助. （KLGE-2022-16）
